TAILIEUCHUNG - The use of random amplified polymorphic DNA (RAPD) markers in sex discrimination in Nile tilapia, Oreochromis niloticus (Pisces: Cichlidae)
Random amplified polymorphic DNA (RAPD) markers were successfully used in discrimination of sexes in Nile tilapia fish (Oreochromis niloticus) using linear discriminant function analysis. The results provide support for the view that major genetical sex determining factors exist in tilapia. | Turk J Biol 24 (2000) 169–175 © TÜBİTAK The Use of Random Amplified Polymorphic DNA (RAPD) Markers in Sex Discrimination in Nile Tilapia, Oreochromis niloticus (Pisces: Cichlidae) Fevzi BARDAKCI School of Biological Sciences, University of Wales, Singleton Park, Swansea SA2 8PP, . Received: Abstract: Random amplified polymorphic DNA (RAPD) markers were successfully used in discrimination of sexes in Nile tilapia fish (Oreochromis niloticus) using linear discriminant function analysis. The results provide support for the view that major genetical sex determining factors exist in tilapia. Key Words: RAPD, DNA, sex discrimination, tilapia, Oreochromis Nil Tilapia Balığının (Pisces: Cichlidae) Cinsiyet Ayırımında Rastgele Çoğaltılmış Polimorfik DNA (RAPD) Markerlerinin Kullanımı Özet: Rastgele çoğaltılmış polimorfik DNA (RAPD) markerleri kullanarak lineer ayrıcı fonksiyon analizi, Nil tilapia balığının (Oreochromis niloticus) cinsiyetlerini ayırmada başarılı olarak kullanıldı. Sonuçlar tilapiada ana genetiksel cinsiyet belirleme faktörlerinin olduğu görüşüne destek sağlamaktadır. Anahtar Sözcükler: RAPD, DNA, cinsiyet ayrımı, tilapia, Oreochromis Introduction Amongst vertebrates, fishes contain the greatest variability in sex determination mechanisms including monofactorial, polyfactorial and environmental control (1). In most cases, genes located on the heteromorphic sex chromosomes play the main role in the expression of sex determination in fishes (2). On the other hand, morphological differentiation of sex chromosomes is not apparent in most fish species studied, thus has been demonstrated cytogenetically in few fish species (3-5). The most recent approach to studying the mechanism of sex determination in fishes is to develop sex-specific molecular markers. So far sex-specific markers have only been developed in the guppy, Poecilia reticulata (6), chinook salmon, Oncorhyncus tshawytscha (7), coho salmon, O. kisutch (8) and Leporinus elongatus .
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