TAILIEUCHUNG - Accumulation of poly β-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources
The accumulation of poly-β-hydroxybutyric acid (PHB) in 27 Streptomyces isolates with different taxonomical properties was investigated. The amount of synthesized PHB was determined as crotonic acid by spectrophotometry. Eighty percent of the tested isolates accumulated PHB between and of dry mycelial weight. | Turk J Biol 26 (2002) 171-174 © TÜB‹TAK Accumulation of Poly-β-Hydroxybutyrate in Streptomyces Species During Growth with Different Nitrogen Sources Aysel U⁄UR, Nurettin fiAH‹N Mu¤la University, Faculty of Arts & Sciences, Department of Biology, TR-48187, Kötekli, Mu¤la - TURKEY Yavuz BEYATLI Gazi University, Faculty of Arts & Science, Department of Biology, Ankara - TURKEY Received: Abstract: The accumulation of poly-β-hydroxybutyric acid (PHB) in 27 Streptomyces isolates with different taxonomical properties was investigated. The amount of synthesized PHB was determined as crotonic acid by spectrophotometry. Eighty percent of the tested isolates accumulated PHB between and of dry mycelial weight. PHB was produced by a selectant of Streptomyces isolates in media containing different nitrogen sources (KNO3, glycine, peptone, proteose peptone, L-asparagine, tryptone and malt extract), each combined with 2 g/l glucose as the carbon source. The most effective PHB production was observed on glycine/glucose medium with a dry mycelial weight. Nitrogen limiting conditions were inhibitory to Streptomyces growth, but stimulated PHB accumulation. Based on the morphological and biochemical test results, selected isolates were identified as Streptomyces halstedii (MU112), S. anulatus (MU117) and S. rochei (MU119). Key Words: Poly-β-hydroxybutyric acid (PHB), Streptomyces, nitrogen sources Farkl› Azot Kaynaklar› ile Büyüme S›ras›nda Streptomyces Türlerinde Poly-β-Hydroxybutyrat›n Birikimi Özet: Farkl› taksonomik özelliklere sahip 27 Streptomyces izolat›nda Poly-β-hydroxybutyric asit (PHB) birikimi araflt›r›ld›. Sentezlenen PHB miktar› krotonik asit olarak spektrofotometre ile belirlendi. Denenen izolatlar›n %80’i PHB yi kuru miselyum a¤›rl›¤›n›n %’s› aras›nda akümüle etti. PHB, seçilmifl Streptomyces izolatlar› taraf›ndan, farkl› azot kaynaklar› (KNO3, glisin, pepton, proteoz pepton, L-asparagin, tripton ve malt ekstrakt) ve karbon kayna¤› .
đang nạp các trang xem trước
