TAILIEUCHUNG - Production of monoclonal antibodies specific for progesterone

Progesterone levels in milk and serum are indicators of pregnancy in cattle. The progesterone level reaches a peak on the 21st and 22nd days of pregnancy. Monoclonal antibodies specific to progesterone could be used for the immunodetection of milk and serum progesterone levels. | Tr. J. of Biology 23 (1999) 393–399 © TÜBİTAK Production of Monoclonal Antibodies specific for Progesterone Fatıma YÜCEL Institute for Genetic Engineering and Biotechnology, TÜBİTAK Marmara Research Center, Gebze-Kocaeli-TURKEY Beyazıt ÇIRAKOĞLU Department of Medical Biology, Medical Faculty, Marmara University, Haydarpaşa, İstanbul-TURKEY Received: Abstract:Progesterone levels in milk and serum are indicators of pregnancy in cattle. The progesterone level reaches a peak on the 21st and 22nd days of pregnancy. Monoclonal antibodies specific to progesterone could be used for the immunodetection of milk and serum progesterone levels. We report here the development of hybrid cells prdoducing monoclonal antibodies specific for progesterone using hybridoma technology. Hybridoma cells secreting monoclonal antibodies against progesterone (MAM 2H11, MAM 6G6) were developed by fusion of the spleen cells of an immunized BALB/c mouse with FO (ATCC CRL 1646) mouse myeloma cells. These monoclonal antibodies were characterized for use in the development of diagnostic kits based on the enzyme-linked immunosorbent assay (ELISA) test system. ELISA tests showed that antibodies produced by 2 hybrids from 375 wells reacted specifically with progesterone. Monoclonal antibodies did not show any cross reaction with other steroid hormones. Key Words: Immunodiagnosis, monoclonal antibody, progesterone, ELISA Progesteron’a Özgü Monoklonal Antikorların Üretilmesi Özet: Progesteron hormonunun süt ve serumdaki miktarı gebeliğin bir göstergesidir. İneklerde gebeliğin 21. ve 22. günlerinde progesteron miktarı yüksektir. Progesterona karşı geliştirilmiş monoklonal antikorlar ile süt ve serumdaki progesteron düzeyi belirlenebilmektedir. Bu çalışmada hibridoma teknolojisi kullanılarak progesterona özgün monoklonal antikor üreten hibrit hücrelerinin elde edilmesi amaçlanmıştır. Progesterona karşı monoklonal antikorlar sentezleyen hibridoma klonları (MAM 2H11, MAM 6G6), FO (ATCC CRL .

• Sinh học
• Monoclonal antibodies specific
• Serum progesterone
• Hybrid cells
• Hybridoma technology
• Enzyme linked immunosorbent assay
• BMC Biotechnology
• Humanized rats
• Human antibodies
• Monoclonal antibodies
• Human idiotypes
• Comparative efficacy
• Different methods
• Generation of 12S particles
• 146S particles of FMDV serotype
• Specific monoclonal antibodies
• Hybrid cell
• Monoclonal antibody
• Steroid hormones
• Serum albumine
• BMC Developmental Biology
• Monoclonal antibody screen
• Immunological resources
• Molecular mechanisms
• Schmidtea mediterranea
• tài liệu báo cáo nghiên cứu khoa học
• cách trình bày báo cáo
• kiến thức bệnh thú y
• báo cáo bệnh thú y
• các nghiên về bệnh thú ý
• BMC Veterinary Research
• Porcine reproductive
• Respiratory syndrome virus
• M protein
• Synthetic peptide
• Bayesian model
• Capsid protein VP2
• Feline panleukopenia virus
• Hemagglutination inhibition assay
• Panthera tigris
• Assessment of renal function test
• Perimenopausal middle aged women
• Women with hypothyroidism
• Enzymelinked immunosorbent assay
• Thyroid hormones including thyroid