TAILIEUCHUNG - Primula veris plants derived from in vitro cultures and from seeds: genetic stability, morphology, and seed characteristics
Flow cytometry measurement confirmed the genetic stability of Primula veris L. (cowslip) plants obtained during the micropropagation process. The mean 2C DNA content was pg/2C and P. veris can be classified in the group of plants of very small genomes. | Turkish Journal of Botany Turk J Bot (2018) 42: 412-422 © TÜBİTAK doi: Research Article Primula veris plants derived from in vitro cultures and from seeds: genetic stability, morphology, and seed characteristics 1, 1 2 3 Iwona JEDRZEJCZYK * , Maria MOROZOWSKA , Renata NOWIŃSKA , Andrzej M. JAGODZIŃSKI 1 Laboratory of Molecular Biology and Cytometry, Department of Agricultural Biotechnology, UTP University of Science and Technology, Bydgoszcz, Poland 2 Department of Botany, Poznań University of Life Sciences, Poznań, Poland 3 Institute of Dendrology, Polish Academy of Sciences, Kórnik, Poland Received: Accepted/Published Online: Final Version: Abstract: Flow cytometry measurement confirmed the genetic stability of Primula veris L. (cowslip) plants obtained during the micropropagation process. The mean 2C DNA content was pg/2C and P. veris can be classified in the group of plants of very small genomes. Comparative morphological analysis of cowslip plants regenerated in vitro and derived from seeds was carried out in three subsequent years under common garden conditions, which limited the effects of environmental factors. The origin of specimens significantly influenced most of the morphological traits examined. Plants that originated from seeds were characterized by more intensive development rate and higher total seed production compared to micropropagated plants. Significant differences between the two groups of specimens were observed for the number of flower stalks, flowers, fruits, and seeds per plant. No interseasonal variation within the reproductive traits such as number of seeds per flower stalk and per plant was found for plants derived from in vitro cultures. That allows prediction of seed production from in vitro regenerated plants after their successful acclimatization to field conditions. Importantly, plants derived from in vitro cultures set .
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