TAILIEUCHUNG - Determination of mutagenic potencies of aqueous extracts of Thermopsis turcica by Ames test

Termopsis turcica is an endemic species of Turkey popularly known as Eber Sarısı or Piyam; it is a member of the Th ermopsis genus, which contains plants considered to be medicinal throughout the world. | R. LİMAN, Y. EREN, D. AKYIL, M. KONUK Turk J Biol 36 (2012) 85-92 © TÜBİTAK doi: Determination of mutagenic potencies of aqueous extracts of Thermopsis turcica by Ames test Recep LİMAN, Yasin EREN, Dilek AKYIL, Muhsin KONUK Biology Department, Faculty of Science and Literature, Afyon Kocatepe University, 03200, Afyonkarahisar - TURKEY Received: Abstract: Thermopsis turcica is an endemic species of Turkey popularly known as Eber Sarısı or Piyam; it is a member of the Thermopsis genus, which contains plants considered to be medicinal throughout the world. In this study, the mutagenic effect of aqueous extracts obtained from the leaves, root, stem, and flowers of T. turcica were evaluated by the Ames test, using Salmonella typhimurium TA97, TA98, TA100, and TA102 strains with and without an S9 activation system. The results obtained were analyzed statistically using SPSS for Windows, and the Mann-Whitney test was performed for comparisons. The results showed that all of the aqueous extracts of T. turcica were mutagenic for S. typhimurium TA98 with S9 mix, and, at a dosage of 3750 μg/plate, the leaf extracts were also found to be mutagenic for S. typhimurium TA102 with or without S9 mix. The results further demonstrated that, in relation to the concentration, leaf extracts of T. turcica increased the frequency of reverse mutation in S. typhimurium TA102 with S9 mix. Key words: Ames test, Salmonella typhimurium, mutagenicity Thermopsis turcica sulu ekstrelerinin mutajenik etkilerinin Ames testi ile belirlenmesi Özet: Thermopsis turcica “Eber Sarısı” yada “Piyam” olarak bilinen Türkiye’nin endemik türlerinden birisidir. Thermopsis cinsi bütün dünyada tıbbi bitki olarak kabul edilmektedir. Bu çalışmada, T. turcica’nın yaprak, kök, gövde ve çiceklerinden elde edilen sulu ekstrelerin mutajenik etkisi, Salmonella typhimurium TA97, TA98, TA100 ve TA102 suşları ile S9 varlığında ve yokluğunda Ames testi ile değerlendirildi. Sonuçlar .

• Sinh học
• Ames test
• Salmonella typhimurium
• Nitro o phenylenediamine
• β nicotinamide adenine dinucleotide phosphate
• 2 aminofluorene
• scientific reports
• medical reports
• health education
• tumor cells
• medical knowledge
• chemical reaction
• Bioceramic materials
• Chromosome aberration
• Sister chromatid exchange
• Proliferation index
• Origanum vulgare subsp
• vulgare
• Methanol extract
• AMES Salmonella
• Microsome test system
• S9 metabolic activation system
• α keto acids
• Ames test system
• DNA repair system
• Salmonella mutagenicity