TAILIEUCHUNG - Separation process of rosmarinic acid and their derivatives from celastrus hindsii benth leaves
The optimal conditions for extraction were as followings: solvent/material ratio 12/1 (v/m), two time extractions, and total extraction time of 3 hours. RA extracts were refined by Diaion HP-20column to have RA with % purity and yield % (by dry weight material). | Journal of Science and Technology 54 (2C) (2016) 380-387 SEPARATION PROCESS OF ROSMARINIC ACID AND THEIR DERIVATIVES FROM CELASTRUS HINDSII BENTH LEAVES Ly Ngoc Tram Food Industries Research Institute, 301 Nguyen Trai road, Thanh Xuan, Hanoi, Vietnam Email: tramngocly@ Received: 15 June 2016; Accepted for publication: 27 October 2016 ABSTRACT Rosmarinic acid is a naturally antioxidant, which is biosynthesized from the amino acids Lphenylalanine and L-tyrosine by eight enzymes that include phenylalanine ammonia lyase and cinnamic acid 4-hydroxylase. Rosmarinic acid can also be chemically produced by the esterification of caffeic acid and 3,4-dihydroxyphenyllactic acid. Recently, rosmarinic acid and its derivatives (RA) have attracted interests for their biological activities, which include antiinflammatory, anti-oxidant, anti-angiogenic, anti-tumor, and anti-microbial functions. RA extraction procedure from Xaden leaves Celastrus hindsii was investigated. RA extracts were obtained from 50 % ethanol extract. The optimal conditions for extraction were as followings: solvent/material ratio 12/1 (v/m), two time extractions, and total extraction time of 3 hours. RA extracts were refined by Diaion HP-20column to have RA with % purity and yield % (by dry weight material). Keywords: Xaden, Celastrus hindsii Benth, rosmarinic acid. 1. INTRODUCTION Celastrus hindsii Benth (Chinese bitter-sweet, Xaden) is a species of Celastraceae family, which used to distribute in China, Vietnam, Myanmar, Thailand. In Vietnam, xaden naturally grows in forests, easily growing in the provinces of Hoa Binh, Ha Nam, Quang Ninh, Ninh Binh and is used as a traditional medicine for the treatment of ulcer, tumor, and inflammation. In the search for antitumor agents form Celastraceae family, a number of sesquiterpenoids and triterpenoids have been isolated from the ethanol extract of the dried stems of C. hindsii [1–4]. Among the isolated compounds, maytenfolone-A and celasdine B
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