TAILIEUCHUNG - Radiation synthesis of silver nanoparticles chitosan against Corynespora cassiicola causing leaf fall disease on rubber trees

In this study, silver nanoparticles (AgNPs) were prepared by gamma rays irradiation of , , and 10 mM silver nitrate solution using chitosan as a stabilizer. UV spectra, morphology and size of AgNPs irradiated at different doses were characterized by using UV-vis spectrophotometer and TEM images. | Journal of Science and Technology 55 (1A) (2017) 27-36 DOI: RADIATION SYNTHESIS OF SILVER NANOPARTICLES/CHITOSAN AGAINST Corynespora cassiicola CAUSING LEAF FALL DISEASE ON RUBBER TREES Le Thi An Nhien1, Luong Nguyen Thu Tam2, Duong Hoa Xo3, Le Quang Luan3, *, Nguyen Duc Luong4 1 Dong Nai Biotechnology Center, 1597 Pham Van Thuan Street, Thong Nhat Ward, Bien Hoa City, Dong Nai Province 2 Nong Lam University, Linh Trung Ward, Thu Duc District, Ho Chi Minh City 3 Biotechnology Center of Ho Chi Minh City, 2374 Highway 1, District 12, Ho Chi Minh City 4 Ho Chi Minh City University of Technology, 268 Ly Thuong Kiet Street, Ward 14, District 10, Ho Chi Minh City * Email: lequangluan@ Received: 30 Oct 2016; Accepted for publication: 30 May 2017 ABSTRACT In this study, silver nanoparticles (AgNPs) were prepared by gamma rays irradiation of , , and 10 mM silver nitrate solution using chitosan as a stabilizer. UV spectra, morphology and size of AgNPs irradiated at different doses were characterized by using UV-vis spectrophotometer and TEM images. The obtained results indicated that the average size of AgNPs increased by the increase of silver concentration in irradiated solution or the degree of acetylation of chitosan, while the increase of chitosan concentration was found to be a functional key for reducing the average size of particles in AgNPs product. In vitro test, AgNPs inhibited the growth of Corynespora cassiicola. In particularly, the inhibitory efficiency of AgNPs on the growth of C. cassiicola on rubber leaf extract media increased from to 100 % when the average particle size of particles in AgNPs product decreased from 15 to 5 nm at the concentration of 50 ppm. In addition, the increase of AgNPs concentration from 10 to 90 ppm also enhanced the antifungal activity to be from to 100 %, respectively. It suggests that the silver nanoparticles/chitosan (AgNPs/chitosan) synthesized by γ-rays irradiation

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