TAILIEUCHUNG - In vivo study on ethanolic extract of allium cepa on mice to evaluate the anti hyperglycemic activity
While the normoglycemic test came up with no significant changes on blood glucose levels of the normoglycemic mice, the glucose tolerance test pointed out the extract’s dosage of 4 mg/kg could considerably reduce blood glucose level of oral-glucose-loading mice compared to negative control administering saline water and positive control administering Metformin and Amaryl. Eventually, since the study partially confirmed the anti-hyperglycemic activity of A. cepa ’s extract, further investigations are recommended. | Vietnam Journal of Science and Technology 55 (6A) (2017) 112-119 IN VIVO STUDY ON ETHANOLIC EXTRACT OF ALLIUM CEPA ON MICE TO EVALUATE THE ANTI-HYPERGLYCEMIC ACTIVITY Thanh-Hoang Nguyen-Vo1, Dat Nguyen1, Phuc Le1, Hieu Doan1, Quy Trinh1, Ngan Tran1, Ly Le1, 2, * 1 International University, Vietnam National University, Ho Chi Minh City, Viet Nam 2 Institute for Computational Science and Technology, Ho Chi Minh City, Viet Nam Email: Received: 15 July 2017; Accepted for publication: 15 December 2017 ABSTRACT As diabetes and blood glucose concerns have been raising, other feasible treatments are now being conducted beside the conventional ones. There has been a shift to the consumption of naturally-derived medicines instead of chemically-derived ones due to novel tendency in medical treatment application. Allium cepa (A. Cepa), a familiar herb used in daily life as a spicy in cooking, was found to have anti-hyperglycemic activity. Extraction using various concentration of ethanol was done to assess the efficiency in total extraction. The 0o ethanol solvent (distilled water) was observed to give the highest efficiency in crude extraction. Total flavonoid and total phenolic content was measured. Although the efficiency in crude extraction of 96o ethanol is lower than those of other concentrations, it flavonoids-releasing and phenolicsreleasing capacities are the greatest ones, which are % and % respectively. The IC50 values of 70o 80o, and 90o ethanolic extract are far higher than that of the control. Then, normoglycemic test and glucose tolerance test were conducted to evaluate the antihyperglycemic effect of the A. cepa ’s extract. While the normoglycemic test came up with no significant changes on blood glucose levels of the normoglycemic mice, the glucose tolerance test pointed out the extract’s dosage of 4 mg/kg could considerably reduce blood glucose level of oral-glucose-loading mice compared to negative control administering
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