TAILIEUCHUNG - Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives

Within the genus Solanum, the term ‘eggplant’ encompasses several cultivated species that are used for food and, to a lesser extent, for medicine. The use of one common name to describe more than one species and the existence of many related wild species have led to taxonomic confusion which, in turn, have complicated analyses of evolutionary relationships and genetic diversity within this groups of species. | Y. TÜMBİLEN, A. FRARY, M. C. DAUNAY, S. DOĞANLAR Turk J Biol 35 (2011) 125-136 © TÜBİTAK doi: Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives Yeliz TÜMBİLEN1, Anne FRARY1, Marie Christine DAUNAY2, Sami DOĞANLAR1 1 İzmir Institute of Technology, Department of Molecular Biology and Genetics, 35430 Urla, İzmir - TURKEY 2 INRA, UR1052, Unité de Génétique et d’Amélioration des Fruits et Légumes F-84140, Montfavet - FRANCE Received: Abstract: Within the genus Solanum, the term ‘eggplant’ encompasses several cultivated species that are used for food and, to a lesser extent, for medicine. The use of one common name to describe more than one species and the existence of many related wild species have led to taxonomic confusion which, in turn, have complicated analyses of evolutionary relationships and genetic diversity within this groups of species. A further challenge for eggplant research is that, despite the fact that the use of molecular markers for phylogenetic studies is well-established, very few studies have described the development of new markers for eggplant. In our work, genic microsatellite (SSR) markers were identified from an expressed sequence tag library of S. melongena and used for analysis of 47 accessions of eggplant and closely related species. The markers had very good polymorphism in the 18 species tested including 8 S. melongena accessions. Moreover, genetic analysis performed with these markers showed concordance with previous research and knowledge of eggplant domestication. These markers are expected to be a valuable resource for studies of genetic relationships, fingerprinting, and gene mapping in eggplant. Key words: Genic SSRs, microsatellites, Solanum, S. aethiopicum, S. macrocarpon, S. melongena Patlıcan ve yakın akraba türlerinde genetik çeşitliliği incelemek için EST-SSRs işaretleyicilerinin uygulanması Özet: Solanum cinsi içerisinde, ‘patlıcan’ terimi gıda ve .

• Sinh học
• Genic SSRs
• S
• aethiopicum
• S
• macrocarpon
• S
• melongena
• genic microsatellite
• Molecular markers
• Genetic diversity
• Drought tolerance
• Cluster analysis
• Population structure
• Simple sequence repeats
• Yellow rust
• Genic microsatellite markers
• Wheat genotypes