TAILIEUCHUNG - Chromosomal differentiation between populations of Clarias gariepinus (Burchell, 1822) from the Göksu Delta and Orontes River (Turkey)
The karyotype of Clarias gariepinus was described by means of G-banding, C-banding, Q-banding, and AgNORs (NORs) staining in this study. | Turk J Biol 35 (2011) 79-87 © TÜBİTAK doi: Chromosomal differentiation between populations of Clarias gariepinus (Burchell, 1822) from the Göksu Delta and Orontes River (Turkey) Arzu KARAHAN1, Serap ERGENE2 1 Middle East Technical University, Institute of Marine Science, Department of Biology, Erdemli, Mersin - TURKEY 2University of Mersin, Faculty of Arts and Sciences, Department of Biology, Mersin - TURKEY Received: Abstract: The karyotype of Clarias gariepinus was described by means of G-banding, C-banding, Q-banding, and AgNORs (NORs) staining in this study. Samples were collected from 2 regions of Turkey: the Göksu Delta and Orontes River. Although samples from both regions had the same diploid chromosome number and fundamental arm number, (2n = 56, FN = 100), they had remarkable differences in karyotype formula and distribution of rDNA sites, according to silver nitrate staining and G-banding. While the karyotype formula for Orontes River samples was 24m + 10sm + 10st + 12a, the Göksu Delta samples’ was 28m + 6sm + 10st + 12a. Comparative analysis showed that there was intraspecific variability in NORs and different NOR numbers in the 2 populations. The study’s findings show that the Göksu Delta and Orontes River samples differed from each other. Key words: Clarias gariepinus, Cytogenetic analyses, Göksu Delta, Orontes River, Turkey Göksu Deltası ve Asi Nehri’ndeki Clarias gariepinus (Burchell, 1822) populasyonları arasındaki kromozomal farklılık (Türkiye) Özet: Bu çalışmada Giemsa bant, C-bant, Ag-NOR bant ve Quinacrine bant teknikleri ile Clarias gariepinus’un karyolojik analizi yapılmıştır. Örnekler Türkiye’nin iki farklı bölgesinden yakalandı: Göksu Delta’sı ve Asi Nehri. Her ne kadar her iki bölge örneklerinde diploid kromozom sayısı ve kromozom kol sayıları aynı bulunmuş olsa da (2n = 56, NF = 100) karyotip formüllerinin, gümüş nitrat boyama ile belirlenen rDNA bölgelerinin ve G-bant bölgelerinin oldukça farklı olduğu .
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