TAILIEUCHUNG - Phylogenetic analysis of peste des petits ruminants virus (PPRV) isolated in Iran based on partial sequence data from the fusion (F) protein gene

In order to gain some insight into the origin of the peste des petits ruminants virus (PPRV) isolated in Iran, a selected region of the PPRV genome in a clinical sample collected from a sheep was amplified using RT-PCR, and the resulting amplicon was sequenced for phylogenetic analysis. | Turk J Biol 35 (2011) 45-50 © TÜBİTAK doi: Phylogenetic analysis of peste des petits ruminants virus (PPRV) isolated in Iran based on partial sequence data from the fusion (F) protein gene Majid ESMAELIZAD1, Saber JELOKHANI-NIARAKI1, Rohani KARGAR-MOAKHAR2 1Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj - IRAN 2Department of virology, Razi Vaccine and Serum Research Institute, Karaj - IRAN Received: Abstract: In order to gain some insight into the origin of the peste des petits ruminants virus (PPRV) isolated in Iran, a selected region of the PPRV genome in a clinical sample collected from a sheep was amplified using RT-PCR, and the resulting amplicon was sequenced for phylogenetic analysis. The partial nucleotide and predicted amino acid sequence of the Iranian isolate was aligned with the corresponding sequences of 16 previously published F genes. Sequence analysis of the strains showed that the Iranian isolate had the highest degree of homology with the majority of the strains, with the exception of Nigerian isolates and ICV89. In general, a higher degree of amino acid sequence conservation was observed among the various strains of field PPRV. Phylogenetic comparison of the Iranian isolate, along with some published exotic sequences, indicated that the virus has been circulating for years in Iran’s neighboring countries, including Turkey and Pakistan. Overall analysis of the amino acid and nucleotide substitutions showed that ICV89 from Ivory Coast was more prone to sequence alterations than the others were. The phylogenetic tree created for F protein nucleotide data was divided into 4 separate lineages. All strains were conserved within the cleavage site of the amino acid sequence, except for Mdn96. Analysis of the sequence data showed that PPRV circulation has been homologous in Asian countries. Determination of the nucleotide sequence of the partial F protein of the Iranian isolate would help .

• Sinh học
• Phylogenetic analysis
• Peste des petits ruminants virus
• PPRV genome
• Viral RNA
• Vero cell monolayer cultures
• Mycoplasma gallisepticum
• Molecular and phylogenetic analysis
• Sequencing and phylogenetic analysis
• Inv E gene
• Inv E gene of salmonella typhimurium
• Salmonella typhimurium
• Listeria monocytogenes
• Labeo catla (Catla fish)
• Dasyatis pastinaca (Stingrays fish)
• Genus Cyclotrichium
• Phylogenetic evaluation of Cyclotrichium
• Morphological revision
• Cytogenetic properties
• Molecular identification
• Phylogenetic tree
• PCR amplification
• Phylogenetic diversity analysis
• Ralstonia Solanacearum
• Pseudomonas Fluorescens
• 28S rRNA gene
• Indigenous xiphinema pachydermum
• Phylogenetic trees
• Xiphinema pachydermum
• Strain specific PCR
• Glycoprotein based phylogenetic analysis
• Rabies virus isolates
• Rabies virus in canids
• Glycoprotein sequence based phylogenetic
• BMC Plant Biology
• HMW GS silencing
• Drought stress
• Molecular cloning
• BiP genes
• Triticum aestivum L
• Human transcription
• Phylogenetic relationship
• Few selected human transcription
• Factors sequence analysis
• Computational methods
• BMC Veterinary Research
• Bovine ephemeral fever virus
• Molecular characterization
• Nasal discharge
• Porcine circovirus 3
• Open reading frames
• Viral capsid
• Hepatitis E
• Zoonotic disease
• Pig blood
• Molecular detection
• Lumpy skin disease
• Infectious viral disease
• Roe deer
• Next generation sequencing
• Taxonomic classification
• Molecular relationship
• Foot and mouth disease virus serotype
• Indian vaccine strain
• World by phylogenetic analysis
• Turkish Journal of Botany
• Tạp chí Thực vật học
• Tripleurospermum eskilensis
• Morphological characters
• Molecular phylogenetic analysis
• Gossypium sp
• SSR marker
• Cluster analysis
• Genetic diversity
• Phylogenetic relationships
• Potential cotton
• Pseudoroegneria species
• Chloroplast trnL F sequences
• Chloroplast DNA
• ITS sequences
• MatK sequences
• Molecular phylogenetic
• Onobrychis species
• Onobrychis Mill
• Internal transcribed spacers
• Genus Aeschynomene
• Phylogenetic framework
• Nod dependent
• Nod independent symbioses
• Genetic relationship
• Genetic distance
• Microsatellite markers
• Zebu cattle
• BMC Bioinformatics
• Genome evolution
• Regulatory evolution
• Genomic algorithms
• Ancestral reconstruction
• Salmonella enterica
• 16S RNA
• Biochemical tests
• Salmonella enterica based on 16S RNA
• Taxonomic identification
• Phylogenetic position
• Archaeal organisms
• 16S DNA sequences
• Plant Biology
• Chloroplast genome
• Molecular markers
• Species identification