TAILIEUCHUNG - The application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions

The present study aimed to determine the genetic relationship between Poa angustifolia and Poa trivialis as well as intraspecies genetic diversity using inter simple sequence repeat (ISSR) markers. ISSR amplification was evaluated for its applicability to narrow leaved bluegrass and rough bluegrass accessions identification. | E. ARSLAN, A. TAMKOÇ Turk J Biol 35 (2011) 415-423 © TÜBİTAK doi: The application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions Emine ARSLAN1, Ahmet TAMKOÇ2 1 Department of Biology, Faculty of Science, Selçuk University, 42031 Konya - TURKEY 2 Department of Field Crops, Faculty of Agriculture, Selçuk University, 42031 Konya - TURKEY Received: Abstract: : The present study aimed to determine the genetic relationship between Poa angustifolia and Poa trivialis as well as intraspecies genetic diversity using inter simple sequence repeat (ISSR) markers. ISSR amplification was evaluated for its applicability to narrow leaved bluegrass and rough bluegrass accessions identification. A total of 401 bands, 363 of which were polymorphic (), were generated using selected 20 primers. Genetic similarity between accessions belonging to Poa species was estimated using Nei’s genetic similarity and UPGMA (unweighted pair group method with arithmetic) cluster analysis was used to construct a dendrogram. UBC836 and M6 primers were observed to be the most suitable for distinguishing between species. Because interspecies differences are especially important in plant breeding, M5 and UBC812 primers were ideal in distinction of P. trivialis and P. angustifolia accessions, respectively. Primer UBC856 was determined to be ideal in discrimination both intraspecies and interspecies. The ISSR fingerprinting technique used was confirmed to be a reproducible and sensitive tool for the identification of Poa accessions and for determination of the correct starting material for plant breeding. Key words: Poa angustifolia, Poa trivialis, intersimple sequence repeat (ISSR), genetic relationship, genetic diversity Dar yapraklı salkımotu (Poa angustifolia) ve adi salkımotu (Poa trivialis) aksesyonları arasında genetik akrabalığı ve .

• Sinh học
• Poa angustifolia
• Poa trivialis
• Intersimple sequence repeat
• Genetic relationship
• Genetic diversity
• Genetic variability
• Similarity coefficient
• Soybean genotypes
• Genetic diversity analysis
• Molecular marker based genetic diversity
• Molecular marker
• Maize genetic diversity
• Very easy digestible
• Abiotic factor
• Genetic diversity in sunflower
• Sunflower (Helianthus annuus L )
• Inter varietal hybridization programme
• Assessment of genetic diversity
• Genetic diversity among fenugreek
• Fenugreek genotypes
• ISSR markers
• Genetic improvement
• Sequence related amplified polymorphism
• Simple sequence repeat
• Genetic diversity of watermelon
• Brassica compestris
• Genetic diversity analysis based
• Molecular level
• Onion genotypes through RAPD
• Genetic diversity remarkably
• Genetic diversity in Gangatiri cow
• Gangatiri cow
• Employing RAPD marker
• Threatened cycads (cycadaceae)
• Conservation of genetic diversity
• High propagules
• Levels of genetic diversity
• Inter Intra cluster distance
• Chickpea genotypes
• Genetic diversity in reference set
• Genetic advance
• Rainfed rice
• Rainfed rice genotypes
• Genetic diversity in rainfed rice genotypes
• Bottle gourd
• Genetic diversity in bottle gourd
• Yield improvement
• Diversity analysis
• Genetic diversity analysis in sunflower
• Parental lines
• RAPD markers
• Solanum lycopersicon L
• Genetic diversity studies
• Genetic diversity studies in tomato
• D2 statistics
• Genetic divergence
• Random amplified polymorphic DNA
• Polymerase chain reaction
• Genetic diversity analysis of citrus cultivars
• D2 analysis
• Landraces and salinity
• Saline stress condition
• Genetic diversity in traditional landraces
• Rice for yield
• Principal component analysis
• Principal component analysis approach
• Brinjal (Solanum melongena L
• Pinus kwangtungensis
• Pa Co pine (Pinus kwangtungensis Chun ex Tsiang)
• RAPD and ISSR markers
• Genetic diversity in Pa Co pine
• Thirty five genotypes
• Oilseed brassica species
• Gossypium hirsutum L
• Cotton germplasm
• Agro morphological characterization
• PIC and marker
• Genetic diversity in hybrids of tomato
• Tomato (Solanum lycopersicum L
• SSR markers
• Molecular characterization
• Soybean varieties
• Genetic diversity assessment
• Agarose gel electrophoresis
• ISSR primers
• Susceptible genotypes
• Genetic diversity in upland cotton
• Molecular markers
• Mungbean [Vigna radiata (L ) Wilczek]
• Screening and identification
• Identification of ssr markers
• Genetic diversity for mungbean
• Mahalanobis D2 statistics
• Genetic diversity in okra
• West Godavari district during kharif season
• Twenty nine hybrids