TAILIEUCHUNG - Purification and properties of an endoglucanase from Aspergillus niger VTCC-F021

An extracellular endoglucanase (EG) from Aspergillus niger VTCC-F021 was purified to homogeneity with a yield of . The enzyme had a molecular mass of 31 kDa and a specific activity of U/mg protein. | Turk J Biol 36 (2012) 694-701 © TÜBİTAK doi: Purification and properties of an endoglucanase from Aspergillus niger VTCC-F021 Thi Hoa PHAM, Dinh Thi QUYEN*, Ngoc Minh NGHIEM Institute of Biotechnology, Vietnam Academy of Science and Technology 18 Hoang Quoc Viet Road, Caugiay Distr., 10600 Hanoi − VIETNAM Received: ● Accepted: Abstract: An extracellular endoglucanase (EG) from Aspergillus niger VTCC-F021 was purified to homogeneity with a yield of . The enzyme had a molecular mass of 31 kDa and a specific activity of U/mg protein. Optimum temperature was observed at 55 °C and optimum pH at 5. The enzyme was stable up to 50 °C and from pH 5 to 6 with residual activity >80% and 60%, respectively. The kinetic constants Km and Vmax determined for EG, with carboxyl methyl cellulose as a substrate, were mg CMC/mL and U/mg protein, respectively. EDTA increased EG activity by 35% at 5 mM and decreased activity by 12% at 15 mM. The metal ions Cu2+ and Fe2+ activated EG activity 112%−152% at 5−15 mM. EG showed a high resistance to Tween 20 and Tween 80 at − (w/v) and to ethanol and methanol at 10%−20% (v/v) with a residual activity of >80%. The biochemical properties of EG demonstrated that this enzyme was quite different from those of A. niger strains. These results suggested that EG from A. niger VTCC-F021 could be used to investigate the efficacy of feed enzymes. Key words: Aspergillus niger VTCC-F021, carboxymethyl cellulose, endoglucanase, purification, characterization Introduction Cellulose is a major polysaccharide constituent of plant cell walls and one of the most common and renewable organic compounds in the biosphere. It has been used in the production of paper, clothes, food additives, and ruminant nutrients. Although cellulose is the most abundant carbohydrate on earth, relatively few animals are able to digest this polysaccharide efficiently (1) due to the function .

• Sinh học
• Aspergillus niger VTCC F021
• Hydrolytic enzymes
• Extracellular endoglucanase
• Carboxymethyl cellulose
• Feed enzymes
• Detoxifying microbial enzymes
• Serum biochemistry
• Broiler chicken supplemented
• Ameliorate feed toxins
• Fish meal
• Plant based ingredients
• Enzymes in aqua feed industry
• Potential benefits of enzymes
• Maximum for economical operations
• Plant Biology
• Antioxidant enzymes
• Nitrate reductase
• Oxidative stress
• Superoxide dismutase
• BMC Veterinary Research
• Chlorella vulgaris
• Plasma metabolites
• Liver composition
• Feed ingredient
• Bài viết nghiên cứu khoa học
• Hydrolytic Enzyme
• Aspergillus Spp
• Broiler chickens
• Asperigillus enzymes
• Body weigh gain
• Feed conversion ratio