TAILIEUCHUNG - Evaluation of biological activities of goldmoss stonecrop (Sedum acre L.)

In vitro antioxidant, antimicrobial, and antiproliferative activities of methanol, acetone, and ethyl acetate extracts of Sedum acre L. were determined. Their total phenolic contents as well as the concentrations of flavonoids were also evaluated. | Turk J Biol 36 (2012) 580-588 © TÜBİTAK doi: Evaluation of biological activities of goldmoss stonecrop (Sedum acre L.) Milan STANKOVIĆ, Ivana RADOJEVIĆ, Milena ĆURČIĆ, Sava VASIĆ, Marina TOPUZOVIĆ, Ljiljana ČOMIĆ, Snežana MARKOVIĆ Department of Biology and Ecology, Faculty of Science, University of Kragujevac, 34 000 Kragujevac - SERBIA Received: ● Accepted: Abstract: In vitro antioxidant, antimicrobial, and antiproliferative activities of methanol, acetone, and ethyl acetate extracts of Sedum acre L. were determined. Their total phenolic contents as well as the concentrations of flavonoids were also evaluated. The total phenolic content was determined with Folin-Ciocalteu reagent and it ranged between to mg gallic acid/g. The concentration of flavonoids in various extracts of S. acre was determined by using a spectrophotometric method with aluminum chloride and the obtained results varied from to mg rutin/g. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (μg/mL), and its values ranged from to μg/mL. The highest phenolic content and capacity to neutralize DPPH radicals were found in the acetone extract. In vitro antimicrobial activity was investigated by the microdilution method. Minimum inhibitory concentration and minimum microbicidal concentration were determined. Testing was conducted against 17 microorganisms, including 13 strains of bacteria (standard and clinical strains) and 4 species of fungi. The tested extracts showed significant antibacterial activities against bacteria and weak to selective activities against the tested fungi. Antiproliferative activity of the methanol extract on the HCT-116 cell line was determined by MTT assay. Results showed that S. acre has medium activity on cell proliferation, with IC50 values of for 24 h and for 72 h. Based on these results, S. acre is a potential source of phenols as a .

• Sinh học
• Sedum acre
• Antiproliferative activity
• Biological activities
• Goldmoss stonecrop
• Fetal bovine serum
• BMC Biotechnology
• Extracellular vesicles
• Bovine serum
• Human myoblasts
• Muscle cell
• Gene expression
• Human adipose derived mesenchymal stem cells
• BDKRB1 gene
• Serum free culture medium
• Stable cell culture
• Scientific reports
• scientific research
• biochemistry
• medical knowledge
• medical research
• analysis of cytoplasmic
• Leishmania parasites
• Cell conditioned
• Large scale
• MCF 7 culture increased
• Caspase 3
• Real time polymerase chain reaction
• Wound healing
• Migration assay
• Endothelin 1
• Alveolar macrophage
• Nitric oxide synthase
• Nitric oxide
• Potent vasoconstrictive peptide
• Fetal bovine serum