TAILIEUCHUNG - Genetic variability in Grapevine virus A from Vitis vinifera L. × Vitis labrusca L. in Sichuan, China
Grapevine virus A (GVA) has a quasispecies nature and is closely associated with the rugose-wood disease complex of Vitis vinifera L. In the diff erent growing regions of table grape in Sichuan, southwest China, the incidence of GVA infection was found to be by ELISA detection among 178 grape plants (Vitis vinifera L. × Vitis labrusca L.). | Turk J Biol 36 (2012) 542-551 © TÜBİTAK doi: Genetic variability in Grapevine virus A from Vitis vinifera L. × Vitis labrusca L. in Sichuan, China Jianhui WANG1,2,3, Dehui XI1, Jianjun LIU3,4, Keling CHEN2,3, Hongwen LI2,3, Xiao LIU2,3, Shu YUAN1, Sezai ERCİŞLİ5, Honghui LIN1 1 Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064 - . CHINA 2 Horticulture Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066 - . CHINA 3 Key Laboratory of Horticultural Crops of Biology and Genetic Improvement (Southwest Region), Ministry of Agriculture, Chengdu 610066 - . CHINA 4 Sichuan Academy of Agricultural Sciences, Chengdu 610066 - . CHINA 5 Department of Horticulture, Faculty of Agriculture, Atatürk University, 25240 Erzurum - TURKEY Received: ● Accepted: Abstract: Grapevine virus A (GVA) has a quasispecies nature and is closely associated with the rugose-wood disease complex of Vitis vinifera L. In the different growing regions of table grape in Sichuan, southwest China, the incidence of GVA infection was found to be by ELISA detection among 178 grape plants (Vitis vinifera L. × Vitis labrusca L.). The results of restriction fragment length polymorphism (RFLP) studies on the polymerase chain reaction (PCR) products of a total of 139 plasmids, cloned from 15 GVA isolates, suggested that the GVA isolates contained highly divergent variants. The plasmids from each GVA isolate that yielded different PCR-RFLP profiles were preferentially chosen for sequencing and were designated as variants. Furthermore, a phylogenetic study based on the analysis of the GVA coat protein genes and RNA silencing suppressor genes showed that the 40 variants obtained from the 15 GVA isolates represented 4 clades, designated as molecular groups I, II, III, and IV, respectively. The variant-specific PCR detection results indicated that .
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