TAILIEUCHUNG - Comparison of sea buckthorn genotypes (Hippophae rhamnoides L.) based on RAPD and FAME data

FAME and RAPD profiles were used to examine biochemical and genetic relationships between 10 selected sea buckthorn genotypes found in the same area of Eastern Anatolia. | Turk J Agric For 32 (2008) 363-368 © TÜB‹TAK Comparison of Sea Buckthorn Genotypes (Hippophae rhamnoides L.) Based on RAPD and FAME Data Sezai ERC‹fiL‹1,*, Emine ORHAN1, Nalan YILDIRIM2, Güleray A⁄AR2 1 Atatürk University, Faculty of Agriculture, Department of Horticulture, 25240 Erzurum - TURKEY 2 Atatürk University, Faculty of Arts and Science, Department of Biology, 25240 Erzurum - TURKEY Received: Abstract: FAME and RAPD profiles were used to examine biochemical and genetic relationships between 10 selected sea buckthorn genotypes found in the same area of Eastern Anatolia. Fatty acid composition of sea buckthorn berries was determined using gas chromatography. Fatty acid results showed that there were differences between genotypes in both the percent and presence of fatty acids in the berries. Myristic acid was detected only in berries of the ESB8 and ESB9 genotypes. Nervonic acid was detected only in the ESB5 genotype and linoleic acid was detected only in the ESB4 genotype. RAPD data also showed that the ESB4 genotype was in a group distinct from the others. According to these results, it can be concluded that both the absence of linoleic acid and the use of RAPD data could be useful indicators for the characterization and grouping of sea buckthorn genotypes. Key Words: Biochemical and molecular markers, fatty acids, Hippophae rhamnoides, RAPD Yalanc› ‹¤de Genotiplerinin (Hippophae rhamnoides L.) RAPD ve Ya¤ Asidi Profillerine Göre Karfl›laflt›r›lmas› Özet: Bu çal›flmada Do¤u Anadolu Bölgesinde bulunan seçilmifl 10 adet yalanc› i¤de genotipi ya¤ asiti ve RAPD profillerine göre biyokimyasal ve genetik belirteçler ile karfl›laflt›r›lm›fl ve aralar›ndaki iliflkiler belirlenmeye çal›fl›lm›flt›r. Genotiplerin ya¤ asidi tayini gaz kromato¤rafisi ile belirlenmifl ve genotipler ya¤ asitlerinin meyvelerinde var-yok durumu ve oran› bak›m›ndan farkl›l›klar göstermifllerdir. Myristik asit sadece ESB8 ve ESB9 genotiplerinde, nervonik asit ESB5 genotipinde ve .

• Nông nghiệp
• Comparison of sea buckthorn genotypes
• Biochemical and molecular markers
• Fatty acids
• Hippophae rhamnoides
• Fatty acid methyl ester
• Random amplified polymorphic DNA
• Identification of genetic polymorphism
• Polymerase chain reaction
• Molecular biology techniques
• Sequence characterised amplified regions
• DNA Isolation
• RAPD primers
• Heat tolerance chickpea
• Molecular characterization
• Cicer arietinum L
• Sex discrimination
• Nile tilapia fish
• Oreochromis niloticus
• Genetical sex
• Genetic diversity
• Molecular marker
• Random amplified polymorphic DNA fingerprinting
• Pleurotus strains
• Genetic diversity characterization
• Sodium nitrite
• Assessment of genetic polymorphism
• Genetic polymorphism
• Sugarcane somaclones
• Genomic template stability
• Tolerance index
• Hordeum vulgare L
• Genetic relationship
• HPV 16
• HPV 18
• Cervical cancer patient
• Human papilloma virus isolated
• Turkish Journal of Botany
• Tạp chí Thực vật học
• Long terminal repeat retrotransposons
• DNA methylation status
• Coupled restriction enzyme digestion random amplification
• Cephalaria Schrad
• Cephalaria sumbuliana
• Cephalaria davisiana
• Related taxa
• Amplified fragment length polymorphisms
• Molecular marker systems
• Sequence related amplified polymorphism
• Unweighted pair group method with arithmetic averages
• Sugar beet
• Molecular markers
• Polymorphic primers
• DNA markers
• RAPD markers
• Genetic linkage mapping
• Poncirus trifoliata L
• C
• reticulata Blanco
• tài liệu báo cáo nghiên cứu khoa học
• cách trình bày báo cáo
• kiến thức bệnh thú y
• báo cáo bệnh thú y
• các nghiên về bệnh thú ý
• Đề tài nghiên cứu tế bào gốc
• Tiểu luận công nghệ sinh học
• Đề tài kỹ thuật Rapd
• Báo cáo thực hành hóa sinh
• Luận văn nghiên cứu đa dạng sinh học
• Tiểu luận Kỹ thuật di truyền
• Cotton Gossypium hirsutum L
• Polymerase chain reaction method
• Gossypium genotypes
• Genetic relationships
• Velezia L
• Unweighted pair group method
• RAPD technique
• Plant genetic resources
• RAPD dendrogram
• Phenotypic similarity
• Terrestrial orchids
• Principal component analysis
• Principal coordinate analysis
• Natural source
• Primer combinations
• Green gram genotypes
• Morphological markers
• Simple sequence repeat
• Tạp chí khoa học
• Mối quan hệ di truyền
• Kỹ thuật RAPD
• Quan hệ di truyền
• Đa dạng di truyền
• Giống đậu tương
• DNA đa hình
• Random Amplified Polymorphic
• Nhân bản ngẫu nhiên
• Hệ số tương đồng di truyền
• Chickpea Cicer arietinum L
• Root rot
• Pathogen free seed
• Genetic diversity analysis of citrus cultivars
• Bellis perennis
• RAPD PCR analysis
• Genomic structures of species
• Rhaponticoides iconiensis
• Oxytona Bernh
• Papaver accessions
• Oxytona species
• Polymerase chain reactions