TAILIEUCHUNG - Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta

Molecular techniques are used today in many areas and have increased the confidence level of studies. The aim of the study was to compare the classical morphological characters of coccoid green algae under field and culture conditions by using modern molecular analysis. | E. N. SOYLU, A. GÖNÜLOL Turk J Biol 36 (2012) 247-254 © TÜBİTAK doi: Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta Elif Neyran SOYLU1, Arif GÖNÜLOL2 1 Giresun University, Faculty of Arts and Science, Department of Biology, Giresun - TURKEY 2 Ondokuz Mayıs University, Faculty of Arts and Science, Department of Biology, Samsun - TURKEY Received: Abstract: Molecular techniques are used today in many areas and have increased the confidence level of studies. The aim of the study was to compare the classical morphological characters of coccoid green algae under field and culture conditions by using modern molecular analysis. To achieve this aim, we first isolated the coccoid green algae from Liman and Cernek lakes. The morphological variations of green algae were observed under culture conditions and the phylogenetic relationships of these strains were defined according to the 18S rRNA gene sequences. According to sequence analysis, 2 of the isolated species had high similarity to Scenedesmus subspicatus (98%) and Desmodesmus sp. (100%). Key words: Coccoid green algae, Scenedesmus subspicatus, molecular analysis, Desmodesmus sp., sequence analysis Kızılırmak Deltası’ndaki göllerden izole edilen küresel yeşil alglerin morfolojik ve 18S rRNA analizi Özet: Günümüzde moleküler teknikler pek çok alanda kullanılmakta ve yapılan çalışmaların güvenilirliğini artırmaktadır. Araştırmanın genel amacı, küresel yeşil alglerin klasik morfolojik karakterlerini arazi ve kültür şartları altında modern moleküler analizleri kullanarak karşılaştırmaktır. Bu amacı gerçekleştirebilmek için öncelikle Liman ve Cernek göllerinden (Samsun, Türkiye) alınan küresel yeşil alglerin farklı klon kültürlerinin izolasyonu gerçekleştirilmiştir. Kültür şartları altında yeşil alglerin morfolojik çeşitliliği gözlemlenerek, bu suşların 18S rRNA gen dizilerine göre filogenetik ilişkileri belirlenmiştir. DNA dizi .

• Sinh học
• Coccoid green algae
• Scenedesmus subspicatus
• Molecular analysis
• Desmodesmus sp
• Sequence analysis
• Identification and sequence analysis
• Sequence analysis of a DREB subfamily transcription
• DREB subfamily transcription factor involved
• Drought stress tolerance from rice
• Drought stress tolerance
• EST SSR
• Rosa spp
• Polymorphic information content
• Different varieties of roses
• Expressed sequence tag simple sequence repeat
• Marker resources
• BMC Bioinformatics
• Sequence logo
• Sequence motif
• Position weight matrix
• Binding sites
• Continuous sequence elements
• Nucleic acid sequences
• Flexible method
• Command line based method
• N terminal acetylation
• N terminal acetyltransferase
• Decision tree
• Sequence context
• Short sequence mapping
• Next generation sequencing
• Accurate alignment tools
• RNA Seq data
• DNA isolation
• Herbarium material
• Lichen specimens
• Hexadecyltrimethylammonium bromide
• DsRNA binding protein encoding
• Comparative sequence analysis
• Double stranded RNA
• Binding protein encoding gene
• Indian camels
• Trichoderma isolates
• Tobacco growing regions
• Godavari district
• Based on sequence analysis
• Region of RDNA and morphological variations
• Human transcription
• Phylogenetic relationship
• Few selected human transcription
• Factors sequence analysis
• Computational methods
• Single cell
• B cell
• Software automation
• Protein sequence analysis
• Enzyme activity
• Tertiary structure
• Multiple mutations
• Keratin associated protein
• Keratin associated protein gene
• Two indigenous Pashmina goat breeds
• DNA amplification by Polymerase Chain Reaction
• BMC Genomics
• Genome features
• Phylogenetic analysis
• Molecular marker
• Simple sequence repeats
• ChiB gene
• Biocontrol agents
• Chitinolytic bacteria
• Potential applications
• Alternative chemical
• BMC Genetics
• Sequence data
• Milk yield
• GWAS analysis
• Saanen breeds
• Display specific genetic profiles
• Turkish Journal of Botany
• Tạp chí Thực vật học
• Gene ontology analysis
• The complete chloroplast genome
• Chloroplast simple sequence repeats
• Aquilaria species
• Illumina paired end sequencing
• Analysis of GC content
• Transcriptome sequence
• Coscinium Fenestratum
• Average GC content
• NGS data analysis
• RNA Seq
• Cloud computing
• Parallel computing
• Paired end read analysis
• Single end read analysis
• Pipeline architecture
• Nanobody sequence analysis
• Single domain antibodies
• Institute collection and analysis of Nanobodies
• Various nanobodies
• trình bày báo cáo
• tài liệu báo cáo khoa học
• nghiên cứu y học
• báo cáo y học
• kiến thức y học
• biology
• biological research
• applied biology
• modern biology
• biological engineering research
• Genetic diversity
• Genetic improvement
• Sequence related amplified polymorphism
• Simple sequence repeat
• Genetic diversity of watermelon
• BMC Plant Biology
• Expressed sequence tags
• DNA polymerase
• Gymnosperm genera
• Genome sequence analysis
• Single amino acid variants
• DNA binding
• RNA binding
• Protein protein binding