TAILIEUCHUNG - An Adenovirus Vector-Mediated Reverse Genetics System for Influenza A Virus Generation

 An Adenovirus Vector-Mediated Reverse Genetics System for Influenza A Virus Generation established an alternative reverse genetics system for influenza virus generation by using an adenovirus vector (AdV)  which achieves highly efficient gene transfer independent of cell transfection efficiency. | Vol. 81 No. 17 Journal of Virology Sept. 2007 p. 9556-9559 0022-538X 07 0 doi Copyright 2007 American Society for Microbiology. All Rights Reserved. An Adenovirus Vector-Mediated Reverse Genetics System for Influenza A Virus Generation7 Makoto Ozawa 1 2 Hideo Goto 1 2 Taisuke Horimoto 1 2 and Yoshihiro Kawaoka1 2 3 Division of Virology Department of Microbiology and Immunology Institute of Medical Science University of Tokyo Shirokanedai Minato-ku Tokyo 108-8639 Japan1 Core Research for Evolutional Science and Technology Japan Science and Technology Agency Saitama 332-0012 Japan2 and Department of Pathological Sciences School of Veterinary Medicine University of Wisconsin Madison Madison Wisconsin 537063 Received 14 May 2007 Accepted 18 June 2007 Plasmid-based reverse genetics systems allow the generation of influenza A virus entirely from cloned cDNA. However since the efficiency of virus generation is dependent on the plasmid transfection efficiency of cells virus generation is difficult in cells approved for vaccine production that have low transfection efficiencies . Vero cells . Here we established an alternative reverse genetics system for influenza virus generation by using an adenovirus vector AdV which achieves highly efficient gene transfer independent of cell transfection efficiency. This AdV-mediated reverse genetics system will be useful for generating vaccine seed strains and for basic influenza virus studies. The artificial generation of influenza A virus entirely from cloned cDNA in plasmid-transfected cells the so-called plasmid-based reverse genetics system 1 13 represents an important advance in influenza virology 12 15 . This technology has advanced both basic and applied research on influenza virus most notably the development of vaccine seed strains for highly pathogenic influenza viruses including the currently circulating H5N1 viruses 5 17-20 . Since until recently at least eight plasmids had to be transfected .

• Báo cáo khoa học
• An Adenovirus Vector Mediated Reverse
• Genetics System for Influenza A Virus Generation
• Genetics System
• AdV mediated synthesis of influenza virus RNA
• Plasmids possessing the cDNA
• LR clonase