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An RBCC (RING finger, B-box, and coiled-coil) protein was identified that belongs to the superfamilyof zinc-binding proteins and is specifically expressed in the gill of eel, Anguilla japonica. Euryhaline fishes such as eels canmigrate between freshwater and seawater, which is considered to be accomplished by efficient remodeling of the architecture and function of the gill, a major osmoregulatory organ. | Eur. J. Biochem. 269 6152-6161 2002 FEBS 2002 doi 10.1046 j.1432-1033.2002.03332.x RING finger B-box and coiled-coil RBCC protein expression in branchial epithelial cells of Japanese eel Anguilla japonica Kentaro Mivamoto1. Nobuhiro Nakamura1 Masahide Kashiwagi1 Shinji Honda1 Akira Kato1 Sanae Hasegawa2 Yoshio Takei2 and Shigehisa Hirose1 1Department of Biological Sciences Tokyo Institute of Technology Yokohama Japan 2Ocean Research Institute The University of Tokyo Tokyo Japan An RBCC RING finger B-box and coiled-coil protein was identified that belongs to the superfamily of zinc-binding proteins and is specifically expressed in the gill of eel Anguilla japonica. Euryhaline fishes such as eels can migrate between freshwater and seawater which is considered to be accomplished by efficient remodeling of the architecture and function of the gill a major osmoregulatory organ. To identify molecules involved in such adaptive changes we performed differential display using mRNA preparations from freshwater and seawater eel gills and obtained an RBCC clone among several differentially expressed clones. The clone encoded a protein of 514 amino acid residues with structural features characteristic of the RBCC protein we therefore named it eRBCC e for eel . eRBCC mRNA was specifically expressed in the gills with a greater extent in the gills of freshwater eels. Immunohistochemistry revealed that the expression of eRBCC is confined to particular epithelial cells of the gills including freshwater-specific lamellar chloride cells. The RING finger of eRBCC was found to have a ubiquitin ligase activity suggesting an important regulatory role of eRBCC in the remodeling of branchial cells. Keywords freshwater adaptation gill RBCC protein RING finger ubiquitin ligase. RING finger B-box and coiled-coil RBCC proteins are a group of zinc-binding proteins that belong to the RING finger family. They are so called because they have an N-terminal RING finger motif defined by one histidine
