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Vaults are highly conserved, ubiquitous ribonucleoprotein (RNP) particles with an unidentified function. For the three protein species (TEP1, VPARP, and MVP) and a small RNA that comprises vault, expression of the unique 100-kDa major vault protein (MVP) is sufficient to form the basic vault structure. | ềFEBS Journal Crosstalk between Src and major vault protein in epidermal growth factor-dependent cell signalling Euikyung Kim1 Seunghwan Lee1 Md Firoz Mian2 Sang Uk Yun2 Minseok Song2 Kye-Sook Yi2 Sung Ho Ryu2 and Pann-Ghill Suh2 1 Institue of AnimalMedicine College of Veterinary Medicine Gyeongsang National university Jinju Korea 2 Department of Life Science Pohang University of Science and Technology Pohang Korea Keywords ERK signaling pathway MVP Src Src activity tyrosine phophorylation Correspondence E. Kim Institue of AnimalMedicine College of Veterinary Medicine Gyeongsang NationalUniversity Jinju 660-701 Korea Fax 82 55 751 5803 Tel 82 55 751 5812 E-mail ekim@nongae.gsnu.ac.kr P.-G. Suh Department of Life Science Pohang University of Science and Technology 790-784 Korea Fax 82 54 283 4613 Tel 82 54 279 2293 E-mail pgs@postech.ac.kr Note E. Kim and P.-G. Suh contributed equally to this work. Received 14 November 2005 revised 13 December 2005 accepted 19 December 2005 doi 10.1111 j.1742-4658.2006.05112.x Vaults are highly conserved ubiquitous ribonucleoprotein RNP particles with an unidentified function. For the three protein species TEP1 VPARP and MVP and a small RNA that comprises vault expression of the unique 100-kDa major vault protein MVP is sufficient to form the basic vault structure. To identify and characterize proteins that interact with the Src homology 2 SH2 domain of Src and potentially regulate Src activity we used a pull-down assay using GST-Src-SH2 fusion proteins. We found MVP as a Src-SH2 binding protein in human stomach tissue. Interaction of Src and MVP was also observed in 253J stomach cancer cells. A subcellular localization study using immunofluorescence microscopy shows that epidermal growth factor EGF stimulation triggers MVP translocation from the nucleus to the cytosol and perinuclear region where it colocalizes with Src. We found that the interaction between Src and MVP is critically dependent on Src activity and protein MVP .
