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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Discordance between microvascular permeability and leukocyte dynamics in septic inducible nitric oxide synthase deficient mice. | Available online http ccforum.eom content 11 6 R125 Research Discordance between microvascular permeability and leukocyte dynamics in septic inducible nitric oxide synthase deficient mice Steven M Hollenberg Massimiliano Guglielmi and Joseph E Parrillo Cooper University Hospital Cooper Plaza Camden New Jersey 08103 USA Corresponding author Steven M Hollenberg hollenberg-steven@cooperhealth.edu Received 11 Sep 2007 Revisions requested 5 Oct 2007 Revisions received 6 Nov 2007 Accepted 7 Dec 2007 Published 7 Dec 2007 Critical Care 2007 11 R125 doi 10.1186 cc6190 This article is online at http ccforum.com content 11 6 R1 25 2007 Hollenberg et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Mierovaseular dysfunction causing intravascular leakage of fluid and protein contributes to hypotension and shock in sepsis. We tested the hypothesis that abrogation of inducible nitric oxide synthase iNOS activation would decrease leukocyte rolling leukocyte adhesion and microvascular leakage in sepsis. We compared wild-type mice made septic by cecal ligation and puncture with mice deficient in iNOS. Methods Leukocyte dynamics and microvascular permeability were assessed simultaneously by fluorescence intravital microscopy in the cremaster muscle 15 to 20 hours after induction of sepsis by cecal ligation and puncture in C57Bl 6 mice. Rolling and adhesion of leukocytes labeled with rhodamine and leakage of fluorescein isothiocyanate-conjugated albumin was measured in single nonbranching venules 25 to 40 pm and compared among septic wild-type septic iNOS-deficient transgenic and sham-operated control mice. Results Leukocyte rolling and adhesion were increased in septic animals 61.6 14.4 cells minute and .
