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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Gene promoter methylation assayed in exhaled breath, with differences in smokers and lung cancer patients. | Respiratory Research BioMed Central Open Access Gene promoter methylation assayed in exhaled breath with differences in smokers and lung cancer patients Weiguo Han1 5 Tao Wang1 Andrew A Reilly2 Steven M Keller4 and Simon D Spivack 1 3 5 6 Address Wadsworth Center Human Toxicology Molecular Epidemiology Albany NY USA 2Biostatistics NYS Dept of Health Albany NY USA 3Pulmonary Critical Care Medicine Albany Medical College Bronx NY USA 4Thoracic Surgery Albert Einstein College of Medicine Bronx NY USA 5Pulmonary Medicine Albert Einstein College of Medicine Bronx NY USA and 6Depts. of Epidemiology and Genetics Albert Einstein College of Medicine Bronx NY USA Email Weiguo Han - whan@aecom.yu.edu Tao Wang - taowang@aecom.yu.edu Andrew A Reilly - aar@wadsworth.org Steven M Keller - skeller@montefiore.org Simon D Spivack - sspivack@aecom.yu.edu Corresponding author Published 25 September 2009 Received 12 June 2009 Respiratory Research 2009 10 86 doi l0.ll86 l465-992l-l0-86 Accepted 25 September 2009 This article is available from http respiratory-research.cOm content l0 l 86 2009 Han et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background There is a need for new noninvasive risk assessment tools for use in lung cancer population screening and prevention programs. Methods To investigate the technical feasibility of determining DNA methylation in exhaled breath condensate we applied our previously-developed method for tag-adapted bisulfite genomic DNA sequencing tBGS for mapping of DNA methylation and adapted it to exhaled breath condensate EBC from lung cancer cases and non-cancer controls. Promoter methylation patterns were analyzed in DAPK RASSFIA and PAX5P promoters in EBC samples from 54 individuals comprised of
