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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" " Mitogen-activated protein kinases and NFκB are involved in SP-A-enhanced responses of macrophages to. | Respiratory Research BioMed Central Open Access Mitogen-activated protein kinases and NFkB are involved in SP-A-enhanced responses of macrophages to mycobacteria Joseph P Lopez2 David J Vigerust3 and Virginia L Shepherd 1 2 Address Department of Veterans Affairs Medical Center Nashville TN USA 2Department of Pathology Vanderbilt University Nashville TN USA and 3Department of Pediatrics Vanderbilt University Nashville TN USA Email Joseph P Lopez - joe.lopez@vanderbilt.edu David J Vigerust - dave.vigerust@vanderbilt.edu Virginia L Shepherd - virginia.l.shepherd@vanderbilt.edu Corresponding author Published I July 2009 Respiratory Research 2009 10 60 doi I0.II86 I465-992I-I0-60 Received I0 February 2009 Accepted I July 2009 This article is available from http respiratory-research.cOm content I0 I 60 2009 Lopez et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Surfactant protein A SP-A is a C-type lectin involved in surfactant homeostasis as well as host defense in the lung. We have recently demonstrated that SP-A enhances the killing of bacillus Calmette-Guerin BCG by rat macrophages through a nitric oxide-dependent pathway. In the current study we have investigated the role of tyrosine kinases and the downstream mitogen-activated protein kinase MAPK family and the transcription factor NFkB in mediating the enhanced signaling in response to BCG in the presence of SP-A. Methods Human SP-A was prepared from alveolar proteinosis fluid and primary macrophages were obtained by maturation of cells from whole rat bone marrow. BCG-SP-A complexes were routinely prepared by incubation of a ratio of 20 pg of SP-A to 5 X IQS BCG for 30 min at 37 C. Cells were incubated with PBS SP-A BCG or SP-A-BCG complexes for .
