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Olfaction is primarily mediated by the large family of olfactory receptors. Although all insect olfactory receptors share the same structure with seven transmembrane domains, they present poor sequence homologies within and between species. As the only exception, Drosophila melanogaster OR83b and its orthologues define a receptor subtype singularly conserved between insect species. | ỊFEBS Journal Identification of an atypical insect olfactory receptor subtype highly conserved within noctuids Isabelle Brigaud1 Nicolas Montagne2 Christelle Monsempes1 Marie-Christine Francois1 and Emmanuelle Jacquin-Joly1 1 INRA UMR PISC UMR-A 1272 Versailles France 2 UPMC Universite Paris 6 UMR PISC Paris France Keywords Lepidoptera Noctuidae olfaction olfactory receptor phylogeny Correspondence E. Jacquin-Joly INRA UMR 1272 INRA-UPMC PISC Physiologie de l Insecte Signalisation et Communication route de Saint-Cyr F-78000 Versailles France Fax 33 1 30 83 31 19 Tel 33 1 30 83 32 12 E-mail jacquin@versailles.inra.fr Received 24 June 2009 revised 31 July 2009 accepted 3 September 2009 doi 10.1111 j.1742-4658.2009.07351.x Olfaction is primarily mediated by the large family of olfactory receptors. Although all insect olfactory receptors share the same structure with seven transmembrane domains they present poor sequence homologies within and between species. As the only exception Drosophila melanogaster OR83b and its orthologues define a receptor subtype singularly conserved between insect species. In this article we report the identification of a new subtype of putative olfactory receptors exceptionally conserved within noct-uids a taxonomic group that includes crop pest insects. Through homology-based molecular cloning homologues of the previously identified OR18 from Heliothis virescens were identified in the antennae of six noc-tuid species from various genera presenting an average of 88 sequence identity. No orthologues were found in genomes available from diverse insect orders and selection pressure analysis revealed that the noctuid OR18s are under purifying selection. The OR18 gene was studied in details in the cotton leafworm Spodoptera littoralis where it presented all the characteristic features of an olfactory receptor encoding gene its expression was restricted to the antennae with expression in both sexes its developmental expression pattern was .
