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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Exogenous glucosamine globally protects chondrocytes from the arthritogenic effects of IL-1β. | Available online http arthritis-research.eom content 8 6 R1 73 Research article Exogenous glucosamine globally protects chondrocytes from the arthritogenic effects of IL-1p Jean-Noel Gouze1 Elvire Gouze1 Mick P Popp2 Marsha L Bush1 Emil A Dacanay1 Jesse D Kay1 Padraic P Levings1 Kunal R Patel1 Jeet-Paul S Saran1 Rachael S Watson1 and Steven C Ghivizzani1 Open Access 1 Department of Orthopaedics and Rehabilitation Gene Therapy Laboratory University of Florida College of Medicine PO Box 1 00137 Gainesville FL 32610-0137 UsA interdisciplinary Center for Biotechnology Research University of Florida Gainesville FL 32610-0137 USA Corresponding author Jean-Noel Gouze gouzej@ortho.ufl.edu Received 27 May 2006 Revisions requested 28 Jun 2006 Revisions received 19 Sep 2006 Accepted 16 Nov 2006 Published 16 Nov 2006 Arthritis Research Therapy 2006 8 R173 doi 10.1186 ar2082 This article is online at http arthritis-research.com content 8 6 R173 2006 Gouze et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The effects of exogenous glucosamine on the biology of articular chondrocytes were determined by examining global transcription patterns under normal culture conditions and following challenge with IL-1p. Chondrocytes isolated from the cartilage of rats were cultured in several flasks either alone or in the presence of 20 mM glucosamine. Six hours later one-half of the cultures of each group were challenged with 10 ng ml IL-1 p. Fourteen hours after this challenge RNA was extracted from each culture individually and used to probe microarray chips corresponding to the entire rat genome. Glucosamine alone had no observable stimulatory effect on the transcription of primary cartilage matrix genes such as aggrecan collagen .
