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Cadmium ions are a potent carcinogen in animals, and cadmium is a toxic metal of significant environmental importance for humans. Response curves were used to investigate the effects of cadmium chloride on the growth of Camplyobacter jejuni. In vitro, the bacterium showed reduced growth in the presence of 0.1 mmcadmium chloride, and the metal ions were lethal at 1 mmconcentration. | ỊFEBS Journal Molecular responses of Campylobacter jejuni to cadmium stress Nadeem O. Kaakoush1 Mark Raftery2 and George L. Mendz3 1 Schoolof MedicalSciences University of New South Wales Sydney Australia 2 BiologicalMass Spectrometry Facility University of New South Wales Sydney Australia 3 Schoolof Medicine Sydney University of Notre Dame Australia Sydney Australia Keywords cadmium detoxification Campylobacter jejuni citrate cycle glutathione thioredoxin reductase Correspondence G. L. Mendz Schoolof Medicine Sydney University of Notre Dame Australia Sydney NSW 2010 Australia Fax 61 293577680 Tel 61 282044457 E-mail GMendz@nd.edu.au Received 30 May 2008 revised 9 July 2008 accepted 11 August 2008 doi 10.1111 j.1742-4658.2008.06636.x Cadmium ions are a potent carcinogen in animals and cadmium is a toxic metal of significant environmental importance for humans. Response curves were used to investigate the effects of cadmium chloride on the growth of Camplyobacter jejuni. In vitro the bacterium showed reduced growth in the presence of 0.1 mM cadmium chloride and the metal ions were lethal at 1 mM concentration. Two-dimensional gel electrophoresis combined with tandem mass spectrometry analysis enabled identification of 67 proteins differentially expressed in cells grown without and with 0.1 mM cadmium chloride. Cellular processes and pathways regulated under cadmium stress included fatty acid biosynthesis protein biosynthesis chemotaxis and mobility the tricarboxylic acid cycle protein modification redox processes and the heat-shock response. Disulfide reductases and their substrates play many roles in cellular processes including protection against reactive oxygen species and detoxification of xenobiotics such as cadmium. The effects of cadmium on thioredoxin reductase and disulfide reductases using glutathione as a substrate were studied in bacterial lysates by spectrophotometry and nuclear magnetic resonance spectroscopy respectively. The presence of 0.1 mM .
