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Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Use of a novel cell-based fusion reporter assay to explore the host range of human respiratory syncytial virus F protein | Virology Journal BioMed Central Methodology Open Access Use of a novel cell-based fusion reporter assay to explore the host range of human respiratory syncytial virus F protein Patrick J Branigan Changbao Liu Nicole D Day Lester L Gutshall Robert T Sarisky and Alfred M Del Vecchio Address Infectious Diseases Research Centocor Inc. 145 King of Prussia Road Radnor PA 19087 USA Email Patrick J Branigan - Pbraniga@cntus.jnj.com Changbao Liu - Cliu12@cntus.jnj.com Nicole D Day - Nday2@cntus.jnj.com Lester L Gutshall - Lgutshal@cntus.jnj.com Robert T Sarisky - Rsarisky@cntus.jnj.com Alfred M Del Vecchio - Adelvecc@cntus.jnj.com Corresponding author Published 13 July 2005 Received 09 June 2005 Virology Journal 2005 2 54 doi 10.1186 1743-422X-2-54 Accepted 13 July 2005 This article is available from http www.virologyj.cOm content 2 1 54 2005 Branigan et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Human respiratory syncytial virus HRSV is an important respiratory pathogen primarily affecting infants young children transplant recipients and the elderly. The F protein is the only virion envelope protein necessary and sufficient for virus replication and fusion of the viral envelope membrane with the target host cell. During natural infection HRSV replication is limited to respiratory epithelial cells with disseminated infection rarely if ever occurring even in immunocompromised patients. However in vitro infection of multiple human and non-human cell types other than those of pulmonary tract origin has been reported. To better define host cell surface molecules that mediate viral entry and dissect the factors controlling permissivity for HRSV we explored the host range of HRSV F protein mediated fusion. Using a novel .
