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Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: In vitro Douglas fir pollen germination: influence of hydration, sucrose and polyethylene. | 11 Ann. For. Sci. 56 1999 11-18 Inra Elsevier Paris Original article In vitro Douglas fir pollen germination influence of hydration sucrose and polyethylene glycol Nicole Dumont-BéBoux Bradley Anholt Patrick von Aderkas Department of Biology Centre for Forest Biology University of Victoria Box 3020 STN CSC Victoria BC V8W 3N5 Canada Received 27 October 1997 accepted 15 May 1998 Abstract - Douglas fir Pseudotsuga menziesii Mirb. Franco pollen stored at low moisture content experiences imbibition shock when put directly onto culture media. This can be overcome by rehydrating the pollen in 100 relative humidity prior to culturing. The long-term effect of rehydration on pollen tube growth was investigated. The impact that osmoticants such as sucrose and polyethylene glycol PEG have on pollen elongation and tube formation was also studied using culture media of increasing osmotic potentials obtained with different sucrose PEG ratios. Rehydration not only improved survival and pollen development on all media but also enhanced pollen tube induction and growth on most of them. Douglas fir pollen produced tubes over a wide range of osmotic potentials -0.73 to -1.88 MPa . Depending on the sucrose PEG ratio between 5 and 55 of grains produced tubes. There was also a media effect on tube morphology. Inra Elsevier Paris. Pseudotsuga menziesii pollen I in vitro germination osmotic potential polyethylene glycol sucrose Resume - Germination in vitro du pollen de douglas influence de 1 hydratation du sucre et du polyethylene glycol. Le pollen de douglas Pseudotsuga menziesii Mirb. Franco conserve sous forme déshydratée subit un choc hydrique lorsqu il est mis en culture. Une réhydratation préalable en atmosphere saturée d eau supprime ce choc. Nous avons étudié 1 effet à long terme d une réhy-dratation douce sur le développement et la croissance des tubes polliniques. L effet du potentiel osmotique du milieu de culture et celui d agents osmotiques tels que le saccharose et le .
