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Handbook of Microbiological Media, Fourth Edition part 100

Handbook of Microbiological Media, Fourth Edition part 100 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | M16 Agar 985 Preparation of Artificial Seawater Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Modified Hutner s Basal Salts Composition per liter MgSO4-7H2O.29.7g Nitrilotriacetic acid.10.0g CaCl2-2H2O.3.34g FeSO4-7H2O.99.0mg NH4 2MoO4.9.25mg Metals 44 .50.0mL Preparation of Modified Hutner s Basal Salts Add nitrilotriacetic acid to 500.0mL of distilled deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled deionized water to 1.0L. Metals 44 Composition per 100.0mL ZnSO4-7H2O.1.1g FeSO4-7H2O.0.5g EDTA.0.25g MnSO4-7H2O.0.154g CuSO4-5H2O.0.04g Co NO3 26H2O.0.025g Na2B4O7-10H2O.0.018g Preparation of Metals 44 Add components to distilled deion-ized water and bring volume to 100.0mL. Mix thoroughly. Vitamin Solution Composition per liter D-Calcium pantothenate.5.0mg Riboflavin.5.0mg Thiamine-HCl.5.0mg Biotin.2.0mg Folic acid. 2.0mg Vitamin B12.0.1mg Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except modified Hutner s basal salts to distilled deionized water and bring volume to 980.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Aseptically add 20.0mL of sterile modified Hutner s basal salts. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation and maintenance of Verrucomicrobium spino-sum. M14 Medium Composition per liter Yeast extract.1.0g D-Glucose.1.0g Tris hydroxymethyl aminomethane.0.753g Artificial seawater.250.0mL Modified Hutner s basal salts.20.0mL pH 7.5 0.2 at 25 C Artificial Seawater Composition per liter NaCl.23.48g MgCl2.4.98g Na2SO4.3.92g CaCl2.1.1g KCl.0.66g NaHCO3.0.19g H3BO3.0.026g SrCl2.0.024g KBr . 6.0mg NaF . 3.0mg Preparation of Artificial Seawater Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Modified Hutner s .