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Handbook of Microbiological Media, Fourth Edition part 16

Handbook of Microbiological Media, Fourth Edition part 16 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Arginine Dihydrolase HiVeg Broth 145 Eagle s Basal Medium Composition per liter HEPES jV-2-Hydroxyethylpiperazine-jV -2-ethanesulfonic acid buffer.9.53g NaCl.6.8g NaHCO3.2.2g Glucose.1.0g KCl.0.4g CaCl2-2H2O.0.2g NaH2PO4.0.125g MgSO4-7H2O.0.1g L-Isoleucine.0.026g L-Leucine.0.026g L-Lysine.0.026g L-Threonine.0.024g L-Valine.0.0235g L-Tyrosine.0.018g L-Arginine.0.0174g L-Phenylalanine.0.0165g L-Cystine.0.012g L-Histidine.8.0mg L-Methionine.7.5mg L-Tryptophan.4.0mg Inositol.1.8mg Biotin.1.0mg Calcium pantothenate.1.0mg Choline chloride.1.0mg Folic acid.1.0mg Nicotinamide.1.0mg Pyridoxal-HCl.1.0mg Thiamine-HCl.1.0mg Riboflavin.0.1mg pH 7.2-7.4 at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Eagle s Basal Medium Add components to dis-tilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0 with NaOH. Filter sterilize. Agarose Solution Composition per liter Agarose.20.0g Preparation of Agarose Solution Add agarose to distilled deion-ized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Preparation of Medium To 1.0L of sterile Eagle s basal medium aseptically add 1.0L of sterile cooled agarose solution and 100.0mL of fetal calf serum. Mix thoroughly. Pour into sterile Petri dishes. Use For the cultivation of animal tissue culture cells used for the growth of arenaviruses. Arginine Assay Medium See Amino Acid Assay Medium Arginine Broth BAM M44 Composition per liter L-Arginine.5.0g Peptone or gelysate peptone.5.0g Yeast extract.3.0g Glucose.1.0g Bromcresol Purple.0.02g pH 6.5 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH so that it will be 6.5 0.2 after sterilization. Distribute into 16 x 150mm screw-capped tubes in 5.0mL volumes. Autoclave medium with loosely capped tubes for 10 min at 15 psi pressure-121 C. Screw the caps on tightly for storage .