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Handbook of Microbiological Media, Fourth Edition part 11 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Amygdalin Medium 95 Ampicillin TY Salt Medium Composition per liter NaCl.10.0g Pancreatic digest of casein.10.0g Yeast extract.5.0g Ampicillin solution.10.0mL pH 7.0 0.2 at 25 C Ampicillin Solution Composition per 10.0mL Ampicillin.50.0mg Preparation of Ampicillin Solution Add ampicillin to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except ampicillin solution to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Bring pH to 7.0. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add 10.0mL of sterile ampicillin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation of various antibiotic resistant bacteria including Escherichia coli. AMS Agar Ammonium Mineral Salts Agar Composition per liter Agar.15.0g MgSO4-7H2O.1.0g K2HPO4.0.7g KH2PO4.0.54g NH4Cl.0.5g CaCl22H2O.0.2g FeSO4-7H2O.4.0mg H3BO4.0.3mg CoCl26H2O.0.2mg ZnSO4-7H2O.0.1mg Na2MoO4-2H2O.0.06mg MnCl24H2O.0.03mg NiCl26H2O.0.02mg CuCl22H2O.0.01mg pH 6.8 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Add sterile methanol to a concentration of 0.5 aseptically to cooled basal medium. Use For the cultivation and maintenance of bacteria that can utilize methanol as a carbon source such as Methylobacterium species Meth-ylomonas species and Methylophilus species. AMS Agar without Methanol Ammonium Mineral Salts Agar without Methanol Composition per liter Agar.15.0g MgSO4-7H2O.1.0g K2HPO4.0.7g KH2PO4.0.54g NH4Cl.0.5g CaCl22H2O.0.2g FeSO4-7H2O.4.0mg H3BO4 . 0.3mg CoCl26H2O.0.2mg ZnSO4-7H2O.0.1mg Na2MoO4-2H2O.0.06mg MnCl24H2O.0.03mg NiCl26H2O.0.02mg CuCl22H2O.0.01mg pH 6.8 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to .
