Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Human stefin B, from the family of cystatins, is used as a model amyloido-genic protein in studies of the mechanism of amyloid fibril formation and related cytotoxicity. Interaction of the protein’s prefibrillar oligo-mers⁄aggregates with predominantly acidic phospholipid membranes is known to correlate with cellular toxicity. | ỊFEBS Journal Interaction with model membranes and pore formation by human stefin B - studying the native and prefibrillar states Sabina Rabzelj1 T Gabriella Viero2 i Ion Gutierrez-Aguirre3 Vito Turk1 Mauro Dalla Serra2 Gregor Anderluh3 and Eva Zerovnik1 1 Department of Biochemistry and Molecular Biology Jozef Stefan Institute Ljubljana Slovenia 2 Fondazione Bruno Kessler and CNR-Istituto di Biofisica Povo Trento Italy 3 Department of Biology BiotechnicalFaculty University of Ljubljana Slovenia Keywords amyloid pores amyloid lipid interaction cystatin C EPM1 mutants surface plasmon resonance Correspondence G. Anderluh Department of Biology BiotechnicalFaculty University of Ljubljana Vecna pot 111 1000 Ljubljana Slovenia Fax 386 1 257 33 90 Tel 386 1 423 33 88 E-mail gregor.anderluh@bf.uni-lj.si E. Zerovnik Department of Biochemistry and Molecular Biology JoZef Stefan Institute Jamova 39 1000 Ljubljana Slovenia Fax 386 1 477 39 84 Tel 386 1 477 3753 E-mail eva.zerovnik@ijs.si Present address TBia d.o.o. Ljubljana Slovenia Laboratory of TranslationalGenomics CIBIO - Center for Integrative Biology Mattarello Trento Italy These two authors contributed equally to this work Received 25 January 2008 revised 25 February 2008 accepted 10 March 2008 doi 10.1111 j.1742-4658.2008.06390.x Human stefin B from the family of cystatins is used as a model amyloido-genic protein in studies of the mechanism of amyloid fibril formation and related cytotoxicity. Interaction of the protein s prefibrillar oligo-mers aggregates with predominantly acidic phospholipid membranes is known to correlate with cellular toxicity. In the present study we measured membrane interaction of the prefibrillar and native states for three variants the Y31 isoform studied previously the wild-type protein and the G4R mutant the latter is observed in progressive myoclonus epilepsy of type 1. In addition to using critical pressure and surface plasmon resonance we assessed membrane permeabilization by calcein .
