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Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Differential sister chromatid exchange response in phytohemagglutinin and pokeweed stimulated lymphocytes of goat (Capra hircus L) | Genet Sei Evol 1997 29 185-192 Elsevier INRA 185 Original article Differential sister chromatid exchange response in phytohemagglutinin and pokeweed stimulated lymphocytes of goat Capra hircus L D Di Berardino1 V Jovino1 A Crasto1 MB Lioi2 MR Scarfi3 I Burguete4 1 Department of Animal Science University of Naples Federico IF 80055 Portici Naples 2 Department of Animal Production University of Basilicata Via N Sauro 85 85100 Potenza 3 CNR-IRECE 80124 Naples Italy 4 Department of Animal Production University of Murcia Espinardo 30071 Murcia Spain Received 3 June 1996 accepted 21 January 1997 Summary - A differential sister chromatid exchange SCE cell response was observed between phytohemagglutinin PHA and pokeweed PKW stimulated blood lymphocytes of goat Capra hircus L exposed to final concentrations of 0.1 0.25 0.5 1 2.5 and 5 pg mL of BUdR. At 0.1 pg mL of BUdR the two mitogens gave very similar SCE cell responses the SCE mean values were 3.17 1.93 for PHA and 3.28 1.76 for PKW and the frequency distributions fit very well the Poisson probability function with both mitogens. For 0.25 pg mL and increasing BUdR concentrations SCE cell rates for pokeweed mitogen were significantly higher than those of PHA. At 5 pg ml of BUdR the SCE cell response was 8.68 3.24 for PKW and 6.96 3.45 for PHA and the difference was statistically significant p 0.0001 for both mitogens the SCE cell frequency distributions fit the Poisson probability function only by adopting a Poisson mixture model which takes into account the presence of two different subpopulations of cells. sister chromatid exchange phytohemagglutinin pokeweed Poisson distribution goat Resume Effets differentiels de la phytohémagglutinìne et du phytolaque sur les échanges entre chromatides sceurs chez les lymphocytes de la chèvre. Une réponse differentielle pour le nombre d echanges entre chromatides soeurs a été observée entre les lymphocytes de chèvre Capra Hircus L stimulés par la phytohémagglutinine PHA OU le .
