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Báo cáo sinh học: " The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events | Genetic Vaccines and Therapy BioMed Central Research Open Access The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection MOI for predicting gene transfer events Bing Zhang1 Pat Metharom1 Howard Jullie1 Kay AO Ellem2 Geoff Cleghorn3 Malcolm J West1 and Ming Q Wei 1 Address department of Medicine University of Queensland Prince Charles Hospital Brisbane AUSTRALIA 2Queensland Institute of Medical Research Brisbane AUSTRALIA and department of Paediatrics and Child Health Royal Children s Hospital Brisbane AUSTRALIA Email Bing Zhang - b.zhang@medicine.uq.edu.au Pat Metharom - p.metharom@surf.net.fr Howard Jullie - h.jullie@mailbox.uq.edu.au KayAO Ellem - kayE@qimr.edu.au Geoff Cleghorn - g.cleghorn@mailbox.uq.edu.au Malcolm J West - malcolm.west@mailbox.uq.edu.au Ming Q Wei - d.wei@mailbox.uq.edu.au Corresponding author Published 04 August 2004 Received 24 October 2003 Accepted 04 August 2004 Genetic Vaccines and Therapy 2004 2 6 doi 10.1186 1479-0556-2-6 This article is available from http www.gvt-journal.cOm content 2 1 6 2004 Zhang et al licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Although lentiviral vectors have been widely used for in vitro and in vivo gene therapy researches there have been few studies systematically examining various conditions that may affect the determination of the number of viable vector particles in a vector preparation and the use of Multiplicity of Infection MOI as a parameter for the prediction of gene transfer events. Methods Lentiviral vectors encoding a marker gene were packaged and supernatants concentrated. The number of viable vector particles was determined by in vitro transduction and fluorescent .