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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Myocardium-derived conditioned medium improves left ventricular function in rodent acute myocardial infarction | Leu et al. Journal of Translational Medicine 2011 9 11 http www.translational-medicine.eom content 9 1 11 TRANSLATIONAL MEDICINE RESEARCH Open Access Myocardium-derived conditioned medium improves left ventricular function in rodent acute myocardial infarction 1.2t 3 4t 1.2 1 5 1 Steve Leu Ying-Hsien Kao Cheuk-Kwan Sun Yu-Chun Lin Tzu-Hsien Tsai Li-Teh Chang Sarah Chua Kuo-Ho Yeh 1 Chiung-Jen Wu1 Morgan Fu1 Hon-Kan Yip1 2 Abstract Background We investigated whether myocardium-derived conditioned medium MDCM is effective in preserving left ventricular LV function in a rat acute myocardial infarction AMI model. Methods Adult male Sprague-Dawley SD rats n 36 randomized to receive either left coronary artery ligation AMI induction or thoracotomy only sham procedure were grouped as follows n 6 per group Group I II and III were sham-controls treated by fresh medium normal rat MDCM and infarct-related MDCM respectively. Group IV V and VI were AMI rats treated by fresh medium normal MDCM and infarct-related MDCM respectively. Either 75 pL MDCM or fresh medium was administered into infarct myocardium followed by intravenous injection 3 mL at postoperative 1 12 and 24 h. Results In vitro studies showed higher phosphorylated MMP-2 and MMP-9 but lower a-smooth muscle actin and collagen expressions in neonatal cardiac fibroblasts treated with MDCM compared with those in the cardiac fibroblasts treated with fresh medium all p 0.05 . Sirius-red staining showed larger collagen deposition area in LV myocardium in Group IV than in other groups all p 0.05 . Stromal cell-derived factor-1a and CXCR4 protein expressions were higher in Group VI than in other groups all p 0.05 . The number of von Willebrand factor- and BrdU-positive cells and small vessels in LV myocardium as well as 90-day LV ejection fraction were higher whereas oxidative stress was lower in Group VI than in Group IV and Group V all p 0.05 . Conclusion MDCM therapy reduced cardiac fibrosis and oxidative stress enhanced
