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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Phosphoproteomics: new insights into cellular signaling. | Review Phosphoproteomics new insights into cellular signaling Marc Mumby and Deirdre Brekken Address Department of Pharmacology and the Alliance for Cellular Signaling University of Texas Southwestern Medical Center Dallas TX 75390-9041 USA. Correspondence Marc Mumby. E-mail marc.mumby@utsouthwestern.edu Published 17 August 2005 Genome Biology 2005 6 230 doi l0.ll86 gb-2005-6-9-230 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2005 6 9 230 2005 BioMed Central Ltd Abstract Developments in the field of phosphoproteomics have been fueled by the need simultaneously to monitor many different phosphoproteins within the signaling networks that coordinate responses to changes in the cellular environment. This article presents a brief review of phosphoproteomics with an emphasis on the biological insights that have been derived so far. Although many biochemical mechanisms are involved in cellular signaling reversible phosphorylation of serine threonine and tyrosine residues is the one most commonly used in mammalian cells. Protein kinases are one of the largest gene families in humans and mice accounting for 1.7 of the human genome 1 2 and up to 30 of all proteins may be phosphorylated 3 . Traditional biochemical and genetic analyses of phosphoproteins and of the kinases and phosphatases that modify them have provided a wealth of information about signaling pathways. These approaches which typically focus on one protein at a time are however not readily amenable to understanding the complexity of protein phosphorylation or how individual phosphoproteins function in the context of signaling networks. The availability of genome databases and advancements in analytical technology especially mass spectrometry has made it possible to study many phosphoproteins and phosphorylation sites at once. The term phosphoproteomics describes a sub-discipline of proteomics that is focused on deriving a comprehensive view of the
