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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Cytological profiling: providing more haystacks for chemists’ needles. | Minireview Cytological profiling providing more haystacks for chemists needles Janet Lorang and Randall W King Address Department of Cell Biology Harvard Medical School 240 Longwood Avenue Boston MA 02115 USA. Correspondence Randall W King. E-mail randy_king@hms.harvard.edu Published 29 July 2005 Genome Biology 2005 6 228 doi 10.1186 gb-2005-6-8-228 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2005 6 8 228 2005 BioMed Central Ltd Abstract Conventional high-throughput chemical genetic screening seeks to identify small-molecule inhibitors of a specific protein or pathway. A recent study describes how unbiased screening of cellular morphology followed by affinity purification to identify targets of compounds with interesting effects can lead to the identification of novel inhibitors. Scientists in the pharmaceutical industry are in constant search of new drugs that can activate or inhibit their target molecule of interest. Combinatorial chemistry has allowed the synthesis of huge numbers of new compounds by combining sets of building blocks and researchers are busily sorting through this haystack of drugs for the perfect needle - a drug specific for the intended target. The usual approach to this search is high-throughput screening of a large library of compounds 500 000 to 1 000 000 compounds is typical in an assay for a single specific effect such as inhibition of an enzyme. Although this strategy allows large numbers of molecules to be screened it measures only a limited range of biological effects. The vast majority of compounds screened will fail to show the desired effect and will be discarded as useless. Thus molecules that may have interesting and useful characteristics will be missed if they do not have the specific effect that is being measured in the screen. Screening strategies that measure the effects of compounds on biological pathways rather than single proteins cover a broader subset of .
