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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Comparative evaluation of INNO-LiPA HBV assay, direct DNA sequencing and subtractive PCR-RFLP for genotyping of clinical HBV isolates | Ali et al. Virology Journal 2010 7 111 http www.virologyj.eom content 7 1 111 VIROLOGY JOURNAL SHORT REPORT Open Access Comparative evaluation of INNO-LiPA HBV assay direct DNA sequencing and subtractive PCR-RFLP for genotyping of clinical HBV isolates Maisa M All 1 4 Fuad Hasan2 3 4 Suhail Ahmad1 4 and Widad Al-Nakib1 4 Abstract Genotypes A to H of hepatitis B virus HBV influence liver disease progression and response to antiviral therapy in HBV-infected patients. Several methods have been developed for rapid genotyping of HBV strains. However some of these methods may not be suitable for developing countries. The performance of INNO-LiPA HBV Genotyping assay LiPA direct DNA sequencing and subtractive PCR-RFLP of genotype-specific HBV genome regions were evaluated for accurately determining the HBV genotypes by analyzing sera n 80 samples from chronic HBV patients. Both LiPA and DNA sequencing identified 63 4 and 13 HBV strains as belonging to genotype D genotype A and mixed genotype A and D respectively. On the contrary the PCR-RFLP-based method correctly identified all 4 genotype A but only 56 of 63 genotype D strains. Seven genotype D strains yielded indeterminate results. DNA sequence comparisons showed that a single nucleotide change in the target region generated an additional restriction site for Nla IV that compromised the accuracy of this method. Furthermore all the mixed genotype A and D strains were identified only as genotype A strains. The data show that the PCR-RFLP-based method incorrectly identified some genotype D strains and failed to identify mixed genotype infections while LiPA and DNA sequencing yielded accurate results. Findings Eight distinct genotypes A to H of hepatitis B virus HBV have been identified and their occurrence exhibits distinct preferences for ethnic origin of the patient and or geographic regions of the world 1 2 . Recent studies have shown that HBV genotypes influence liver disease progression selection of mutants and .
