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Low temperature is an important environmental factor affecting the perfor-mance and distribution of plants. During the so-called process of cold acclimation, many plants are able to develop low-temperature tolerance, associated with the reprogramming of a large part of their metabolism. | IFEBS Journal A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana Thomas Nagele Benjamin A. Kandel Sabine Frana Meike MeiBner and Arnd G. Heyer Biologisches Institut Abteilung Pflanzenbiotechnologie Universitat Stuttgart Germany Keywords acclimation dynamics Arabidopsis carbohydrate metabolism freezing tolerance mathematicalmodelling Correspondence T. Nagele Biologisches Institut Abteilung Pflanzenbiotechnologie Universitat Stuttgart Pfaffenwaldring 57 D-70550 Stuttgart Germany Fax 49 711 685 65096 Tel 49 711 685 69141 E-mail Thomas.Naegele@bio.uni-stuttgart.de These authors contributed equally to this work Received 11 August 2010 revised 22 September 2010 accepted 22 November 2010 doi 10.1111 j.1742-4658.2010.07971.x Low temperature is an important environmental factor affecting the performance and distribution of plants. During the so-called process of cold acclimation many plants are able to develop low-temperature tolerance associated with the reprogramming of a large part of their metabolism. In this study we present a systems biology approach based on mathematical modelling to determine interactions between the reprogramming of central carbohydrate metabolism and the development of freezing tolerance in two accessions of Arabidopsis thaliana. Different regulation strategies were observed for a photosynthesis b soluble carbohydrate metabolism and c enzyme activities of central metabolite interconversions. Metabolism of the storage compound starch was found to be independent of accessionspecific reprogramming of soluble sugar metabolism in the cold. Mathematical modelling and simulation of cold-induced metabolic reprogramming indicated major differences in the rates of interconversion between the pools of hexoses and sucrose as well as the rate of assimilate export to sink organs. A comprehensive overview of interconversion rates is presented from which accession-specific regulation
