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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana. | Clinical and Molecular Allergy BioMed Central Research Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana Greg S Westwood1 Shih-Wen Huang2 and Nemat O Keyhani 1 Open Access Address Department of Microbiology and Cell Science University of Florida Gainesville FL 32611 USA and 2Department of Pediatrics University of Florida College of Medicine 32610 USA Email Greg S Westwood - gregwest@ufl.edu Shih-Wen Huang - huangsw@peds.ufl.edu Nemat O Keyhani - keyhani@ufl.edu Corresponding author Published 22 September 2006 Received 01 August 2006 Accepted 22 September 2006 Clinical and Molecular Allergy 2006 4 12 doi l0.ll86 l476-796l-4-l2 This article is available from http www.clinicalmolecularallergy.cOm content 4 l l2 2006 Westwood et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Entomopathogenic fungi such as Beauveria bassiana are considered promising biological control agents for a variety of arthropod pests. Beauveria species however have the potential to elicit allergenic reactions in humans although no specific allergens have been characterized to date. Methods Four putative allergens were identified within B. bassiana expressed sequence tag EST datasets. IgE-reactivity studies were performed using sera from patients displaying mold allergies against recombinant B. bassiana proteins expressed in E. coli. Results Full length cDNA and genomic nucleotide sequences of four potential B. bassiana allergens were isolated. BLASTX search results led to their putative designation as follows Bb-Enol with similarity to fungal enolases Bb-f2 similar to the Aspergillus .
