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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Characterization of chronic HCV infection-induced apoptosis. | Zekri et al. Comparative Hepatology 2011 10 4 http www.comparative-hepatology.eom content 10 1 4 COMPARATIVE HEPATOLOGY RESEARCH Open Access Characterization of chronic HCV infection-induced apoptosis 1 2 1 1 3 Abdel-Rahman N Zekri Abeer A Bahnassy Mohamed M Hafez Zeinab K Hassan Mahmoud Kamel Samah A Loutfy 1 Ghada M Sherif4 Abdel-Rahman El-Zayadi5 and Sayed S Daoud6 Abstract Background To understand the complex and largely not well-understood apoptotic pathway and immune system evasion mechanisms in hepatitis C virus HCV -associated hepatocellular carcinoma HCC and HCV associated chronic hepatitis CH we studied the expression patterns of a number of pro-apoptotic and anti-apoptotic genes Fas FasL Bcl-2 Bcl-xL and Bak in HepG2 cell line harboring HCV- genotype-4 replication. For confirmation we also assessed the expression levels of the same group of genes in clinical samples obtained from 35 HCC and 34 CH patients. Methods Viral replication was assessed in the tissue culture medium by RT-PCR quantitative Real-Time PCR qRT-PCR detection of HCV core protein by western blot and inhibition of HCV replication with siRNA. The expression level of Fas FasL Bcl-2 Bcl-xL and Bak was assessed by immunohistochemistry and RT-PCR whereas caspases 3 8 and 9 were assessed by colorimetric assay kits up to 135 days post infection. Results There was a consistent increase in apoptotic activity for the first 4 weeks post-CV infection followed by a consistent decrease up to the end of the experiment. The concordance between the changes in the expression levels of Fas FasL Bcl-2 Bcl-xL and Bak in vitro and in situ was statistically significant p 0.05 . Fas was highly expressed at early stages of infection in cell lines and in normal control liver tissues followed by a dramatic reduction post-HCV infection and an increase in the expression level of FasL post HCV infection. The effect of HCV infection on other apoptotic proteins started very early post-infection suggesting that hepatitis
