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A set of 18 F1 hybrids developed as a result of line x tester mating design involving six lines and three testers were evaluated in randomized block design in three replications for heterosis in four quality traits. Heterosis to the extent of 24.68, 65.19, 25.90 and 76.21 over mid parent and 9.09, 38.70, 17.07 and 68.57 over better parent was recorded for number of locules per fruit, pericarp thickness, total soluble solids and ascorbic acid content, respectively. Standard heterosis over Lakshmi to the extent of -7.46, 24.56, 35.21 and 89.39 was recorded for number of locules per fruit, pericarp thickness, total soluble solids and ascorbic acid content respectively and over US-618 to the extent of 5.53, 51.33, 28.86 and 68.92 was recorded for number of locules per fruit, pericarp thickness, total soluble solids and ascorbic acid content respectively. The crosses LE-67 × Pusa Gaurav, LE-65 × Pant T-3, LE-62 × Pant T-3 and LE-64 × Pant T-3 for number of locules per fruit, pericarp thickness, total soluble solids and ascorbic acid content were identified as a best heterotic combination, respectively. | Heterosis for quality traits in tomato Solanum lycopersicum L.