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A survey was conducted in some districts of Upper Brahmaputra Valley Zone of Assam for most prevailing diseases in brinjal during the year 2015-16. Apart from the fruit rot disease of brinjal (Solanum melongena L.) caused by Phomopsis, Phytophthora, Alternaria etc., a new pathogen, Phoma exigua was observed. Initial symptom developed on the fruits as minute, slightly sunken spots, which later produced minute black bodies (pycnidia) scattered and immersed in the infected host tissues. The fungus was isolated from the collected samples and the pathogenicity test was proved by following the Koch’s postulates by detached leaf and fruit technique, which is a new method of pathogenicity test adopted. The isolated pathogen was identified as Phoma exigua on the basis of morphological and cultural characteristics of the pathogen. This is the first report of Fruit rot of Brinjal caused by Phoma exigua on Brinjal in Assam, India. | First report of phoma exigua causing fruit rot of Brinjal in Northeast India Assam with a new pathogenicity test method