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Commercially available molluscan shellfish such as oysters and clams were collected monthly along the Rochepark, Thoothukudi and brought to the laboratory in moist condition immediately after harvesting and analysed for the prevalence of virulence genes of V. parahaemolyticus. The meat was carefully shucked (meat and fluid) from a pool of 6 – 8 oysters/clams and used. Prevalence of tdh, trh genes of V. parahaemolyticus was confirmed by PCR as well as Direct plating method (TCBS). The present study revealed that the effect of season on the presence of virulence genes of V. parahaemolyticus was insignificant compared to other reports.The result of this study shows less prevalence of pathogenic V. parahaemolyticus and moderate to high prevalence of non-pathogenic V. parahaemolyticus in both oysters and clams during summer season followed by winter season. It could also be observed that 2% Crassostrea sp. showed positive for pathogenic (tdh) V. parahaemolyticus and 61% Crassostrea sp. and 51.2% of Meretrix sp. from HB showed positive for non-pathogenic V. parahaemolyticus. | Effect of season on the prevalence of virulence genes of v. Parahaemolyticus in molluscan shellfish along the Thoothukudi coast, India
