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The fungal pathogen causing brown leaf spot of mulberry was isolated in axenic form and identified as the genus Paramyrothecium based on cultural and morphological characteristics. Paramyrothecium sp. (isolate MMLS18) was identified as Paramyrothecium roridum (Tode) L. Lombard & Crous (syn. Myrothecium roridum Tode ex. Fr.) on the basis of ITS-5.8S rDNA sequence analysis. P. roridum was closely related (99%) to P. roridum isolates viz., NYQB452 (Raphanus sativus), HXC15051716 and HXC15051715 (Ipomoea aquatica) from China, 784 and KP10087 (Melon) from Brazil and KP10087 (Watermelon) from Pakistan. The P. roridum isolate MMLS18 (Mulberry) from India clustered within the genus Paramyrothecium and formed a clade with its closest phylogenetic relatives. | Phylogenetic analysis of Paramyrothecium roridum causing brown leaf spot of mulberry
