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A study was conducted to evaluate the efficacy of exogenous application of putrescine and spermine (Polyamine) on postharvest physiological and biochemical behavior of banana cv. Grand Naine. Mature unripe bananas were dipped in varied concentrations of putrescine (PUT) (1 mM, 3 mM and 6 mM) and spermine (SPM) (1 μM, 5 μM and 10 μM) aqueous solution and stored at cold storage (13 °C ± 1, 85 % RH). Dipping of bananas in distilled water served as control. The physiological and biochemical parameters were recorded at 7 days interval after subsequent ripening under ambient conditions (7 +18, 14 +12, 21 + 7 and 28 + 5 days). Results revealed that among treatments 5 µM spermine was more effective in reducing the physiological loss in weight (4.06%), fruit softening (991.18g), colour change, delayed the biochemical changes by decreasing the conversion of starch (3.61%) into sugars (16.53%), and reduced the consumption of organic acids which was witnessed by low respiration rate (123.84 ml CO2 /kg/h), highest value for ascorbic acid (13.69 mg/100g) and titratable acidity (0.396%) while maintaining good organoleptic qualities. Thus, the exogenous application of polyamines could be effective as a postharvest tool in maintaining the quality and shelf life of banana cv. Grand Naine. | Putrescine and spermine affects the postharvest storage potential of banana Cv. grand naine