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Semen cryopreservation allows crucial management of animal genetic diversity. However, the freezethaw process causes biochemical and physical alterations, impairing sperm energy-dependent functions. Currently, many chemicals are added to the media to enhance frozen-thawed sperm quality during artificial insemination. | Journal of Biotechnology 15 4 605-615 2017 IMPACT OF AMPK ACTIVATOR METFORMIN ON SPERM QUALITY Nguyen Thi Mong Diep1 Elisabeth Blesbois2 1Quy Nhon University Viet Nam 2National Institute of Agricultural Research Physiology of Reproduction and Behaviors 37380 Nouzilly France To whom correspondence should be addressed. E-mail diepdhqn@ymail.com Received 19.7.2017 Accepted 29.12.2017 SUMMARY Semen cryopreservation allows crucial management of animal genetic diversity. However the freezethaw process causes biochemical and physical alterations impairing sperm energy-dependent functions. Currently many chemicals are added to the media to enhance frozen-thawed sperm quality during artificial insemination. The aims of this study was to determine the effects of Metformin Metf on fresh chicken sperm motility and ability to perform acrosome reaction and to evaluate Metf s effects on the functions of cryopreserved sperm. Chicken semen was diluted and incubated at 35 C in media supplemented with or without different doses of 5 -AMP-Activated Protein Kinase AMPK activator Metf 0 5 to 5 mM . We then looked for the concentration improving the most sperm quality to use it in the cryopreservation media used for chicken sperm. Our results show that 1 mM Metf is the concentration giving the best results regarding sperm quality. AMPKa phosphorylation viability acrosome reaction ability AR and various motility parameters were negatively affected by the freeze-thaw process and that Metf partially restored them. Sperm quality improved mean increased by 23 for motility by 10 for viability as well as AMPKa phosphorylation mean increased by 30 . Moreover fluorescent intensity levels of phospho-AMPK were also stronger with Metf than in the control. These results show that the presence of Metf in fresh semen has a positive impact on the quality of sperm and helps reducing the gradual decline in sperm motility caused by cryopreservation by partially restoring several essential sperm functions .
