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Korean wild soybean seeds examined in this study were rich in malonyl-genistin. An increasing trend for total soy-isoflavone content was observed from the north to the southeast in the geographical distribution sites, which signifies the effect of genotype and collection site. The information generated in this report will greatly aid soybean breeding stratagems aimed at improving soy-isoflavone concentration in soybean seeds. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2018) 42: 248-261 © TÜBİTAK doi:10.3906/tar-1801-95 http://journals.tubitak.gov.tr/agriculture/ Research Article Isoflavone profile diversity in Korean wild soybeans (Glycine soja Sieb. & Zucc.) 1 2 2 2 3 Chigen TSUKAMOTO , Muhammad Amjad NAWAZ , Ayaka KUROSAKA , Bao LE , Jeong Dong LEE , 4 2 5 6 2, Eunho SON , Seung Hwan YANG , Cemal KURT , Faheem Shehzad BALOCH , Gyuhwa CHUNG * 1 Faculty of Agriculture, Iwate University, Iwate, Japan 2 Department of Biotechnology, Chonnam National University, Chonnam, Republic of Korea 3 Division of Plant Biosciences, Kyungpook National University, Daegu, Republic of Korea 4 National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, Republic of Korea 5 Department of Field Crops, Faculty of Agriculture, Çukurova University, Adana, Turkey 6 Department of Field Crops, Faculty of Agriculture and Natural Sciences, Abant İzzet Baysal University, Bolu, Turkey Received: 20.01.2018 Accepted/Published Online: 28.03.2018 Final Version: 07.00.2018 Abstract: Isoflavones prevent the incidence of cancer, reduce cardiovascular problems, and decrease menopausal symptoms. In soybean (Glycine soja Sieb. & Zucc.) seeds, the content is greatly affected by growing conditions. The variations in soy-isoflavone content were investigated by growing 346 Korean wild soybean accessions from natural habitats under the same agroclimatic conditions. Highperformance liquid chromatography was used to measure the profiles of six soy-isoflavones, i.e. glycosides (daidzin, glycitin, genistin) and malonyl glycosides (malonyl-daidzin, malonyl-glycitin, and malonyl-genistin). The soy-isoflavone content of the wild soybeans ranged from 0.5 to 5.51 mg/g with an average of 2.78 mg/g. The highest mean total soy-isoflavone was genistin (1.393 mg/g), followed by daidzin (0.939 mg/g) and glycitin (0.250 mg/g). Korean wild soybean .
