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Classification of strains of Bacillus sphaericus by different statistical methods

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In this study, the sensitivies of 21 strains of Bacillus sphaericus to 20 different antibiotics were determined with the antibiotic disc susceptibility test. By measuring the diameters of the inhibition zones in terms of millimetres, the obtained values were analysed using the SPSS for Windows package and classifications were made. With these analyses, 21 strains were clustered into 5 groups. | Turk J Biol 27 (2003) 171-179 © TÜB‹TAK Classification of Strains of Bacillus sphaericus by Different Statistical Methods Nazime MERCAN Department of Biology, Faculty of Science and Arts, Pamukkale University, K›n›kl›, Denizli - TURKEY Atalay ÇA⁄LAR Department of Statistics, Faculty of Science, Hacettepe University, Beytepe, Ankara - TURKEY Belma ASLIM, Yavuz BEYATLI Department of Biology, Faculty of Science and Arts, Gazi University, Teknikokullar, Ankara - TURKEY Received: 13.09.2002 Abstract: In this study, the sensitivies of 21 strains of Bacillus sphaericus to 20 different antibiotics were determined with the antibiotic disc susceptibility test. By measuring the diameters of the inhibition zones in terms of millimetres, the obtained values were analysed using the SPSS for Windows package and classifications were made. With these analyses, 21 strains were clustered into 5 groups. In our study, strains IAB 59 and 2297 were placed into a separate class. In addition, those antibiotics which caused a differentation in strains and a discrimination of strains according to the level of antibiotic effect were determined. Key Words: Bacillus sphaericus, classification. Farkl› ‹statistiksel Metotlar Kullan›larak Bacillus sphaericus Sufllar›n›n S›n›fland›r›lmas› Özet: Bu çal›flmada Bacillus sphaericus’a ait toplam 21 suflun 20 de¤iflik antibiyoti¤e olan duyarl›l›klar›, antibiyotik disk duyarl›l›k yöntemi ile ölçüldü. ‹nhibisyon zonu çaplar› milimetre cinsinden ölçülerek elde edilen de¤erler, SPSS for Windows paket program› ile analiz edildi ve s›n›fland›rmalar yap›ld›. Analizler sonunda 21 suflun 5 küme oluflturdu¤u belirlendi. Çal›flmam›zda IAB 59 ve 2297 nolu sufllar›n di¤er sufllardan ayr› bir s›n›fta yer ald›¤› saptanm›flt›r. Ayr›ca sufllarda ortaya ç›kan farkl›laflman›n hangi antibiyotiklerden kaynakland›¤› belirlenmifl ve antibiyotiklerin etkinlik derecelerine göre sufllar›n ayr›m›n›n sa¤lanabilece¤i sonucuna var›lm›flt›r. Anahtar Sözcükler: Bacillus .

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