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İzmir Çamaltı Saltern is the biggest seawater-based saltern in Turkey. To date, it has not been investigated extensively for the existence of poly-3-hydroxybutyrate (PHB)-producing species. In this study, 14 extremely halophilic archaea were isolated, purified, and screened for PHB production. | E. TEKİN, M. ATEŞ, Ö. KAHRAMAN Turk J Biol 36 (2012) 303-312 © TÜBİTAK doi:10.3906/biy-1102-16 Poly-3-hydroxybutyrate-producing extreme halophilic archaeon: Haloferax sp. MA10 isolated from Çamaltı Saltern, İzmir Ebru TEKİN, Mustafa ATEŞ, Özge KAHRAMAN Department of Biology, Basic and Industrial Microbiology Section, Ege University, Bornova, 35100 İzmir - TURKEY Received: 24.02.2011 Abstract: İzmir Çamaltı Saltern is the biggest seawater-based saltern in Turkey. To date, it has not been investigated extensively for the existence of poly-3-hydroxybutyrate (PHB)-producing species. In this study, 14 extremely halophilic archaea were isolated, purified, and screened for PHB production. One strain was then selected as the best PHB producer, further cultivated in different PHB media, and compared with a positive control, Haloferax mediterranei ATCC 33500. PHB was extracted from cells and measured with a spectrophotometer, and then the amount of PHB was measured through comparison with standard PHB. The detected high PHB yield was 6.53% of the dry cell weight in the PHB medium supplemented with acetate. Partial 16S rRNA gene sequence analysis showed 99% similarity to Haloferax alexandrinus strain TMT; therefore, the strain was named Haloferax sp. MA10. Haloferax sp. MA10 and Haloferax alexandrinus TMT have some differences in phenotypic and biochemical properties. With this study, the discovery of PHB-producing extreme halophilic archaeon Haloferax sp. MA10 at İzmir’s Çamaltı Saltern is reported for the first time. Key words: Extremely halophilic archaeon, poly-3-hydroxybutyrate (PHB), PHB extraction and quantification, Haloferax sp. MA10, 16S rRNA gene İzmir Çamaltı Tuzlası’ndan izole edilen poli-3-hidroksibütirat üreticisi ekstrem halofilik arkeon: Haloferax sp. MA10 Özet: İzmir Çamaltı Tuzlası, Türkiye’nin deniz suyu kökenli en büyük tuzlası olma özelliğindedir. Günümüze kadar bu bölgede poli-3-hidroksibütirat (PHB) üretici türün varlığı bakımından çok fazla .
